Chemistry Faculty of M.V. Lomonosov Moscow State University 119991 Moscow, Russia.
Anal Chem. 2022 Jun 28;94(25):9201-9207. doi: 10.1021/acs.analchem.2c02208. Epub 2022 Jun 10.
We report on the lactate biosensor with linear calibration range from 0.5 to 100 mM, which encircles possible levels of this metabolite concentration in both human sweat and blood. The linear calibration range at high analyte concentrations, which exceeds the Michaelis constant of lactate oxidase by several orders of magnitude, is provided by an additional perfluorosulfonated ionomer diffusion membrane. In contrast to the known lactate biosensors, which retain their response within less than a couple of hours, the reported system displays 100% response for dozens of hours even upon high analyte concentrations. The biosensors with an additional diffusion-limiting membrane have been validated for lactate detection both in human blood serum and in undiluted human sweat shortly after its secretion. Both linear response in the entire range of blood and sweat lactate concentrations and ultrahigh operational stability would provide the use of the elaborated biosensor in wearable devices for the monitoring of hypoxia.
我们报告了一种线性校准范围为 0.5 至 100mM 的乳酸生物传感器，其涵盖了人汗液和血液中这种代谢物浓度的可能水平。在分析物浓度较高的情况下（超过乳酸氧化酶米氏常数几个数量级），通过附加的全氟磺酸离子聚合物扩散膜提供线性校准范围。与已知的乳酸生物传感器不同，后者的响应时间不到几个小时，而所报道的系统即使在高分析物浓度下也能保持数十个小时的 100%响应。具有附加扩散限制膜的生物传感器已在人血清和分泌后不久的未经稀释的人汗水中对乳酸检测进行了验证。在血液和汗液乳酸浓度的整个范围内的线性响应和超高的操作稳定性将为在可穿戴设备中用于监测缺氧的精心设计的生物传感器的使用提供了可能。
