Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nantong University, Nantong 226001, China.
Aging (Albany NY). 2022 Jun 10;14(11):4699-4713. doi: 10.18632/aging.203474.
This article researched circ_0063804 effects on ovarian cancer (OC) development and resistance to cisplatin, aiming to provide a new target for OC therapy.
A total of 108 OC patients participated in this study. The circle structure of circ_0063804 was investigated using RNase R. Circ_0063804 expression in OC cells were up-regulated or down-regulated by transfection. Cell proliferation was assessed by cell counting kit-8 assay and colony formation assay. Flow cytometry was used to detect apoptosis. OC cells resistance to cisplatin was explored through MTT assay. Luciferase reporter assay was performed. qRT-PCR and Western blot was applied to research genes expression. Xenograft tumor experiment was conducted using nude mice. Ki67 expression in xenograft tumor was detected by immunohistochemistry.
Circ_0063804 expression was up-regulated in OC patients and indicated poor prognosis ( < 0.05). Circ_0063804 had a stable circle structure. Circ_0063804 enhanced proliferation, resistance to cisplatin and reduced apoptosis of OC cells ( < 0.01). miR-1276 was down-regulated in OC patients and sponged by circ_0063804. CLU was directly inhibited by miR-1276 and up-regulated in OC patients. Circ_0063804 exacerbated malignant phenotype and resistance to cisplatin of OC cells by enhancing CLU expression via sponging miR-1276 ( < 0.01). Circ_0063804 silencing inhibited OC cells growth, resistance to cisplatin and Ki67 expression ( < 0.01).
Circ_0063804 promoted OC cells proliferation and resistance to cisplatin by enhancing CLU expression via sponging miR-1276.
本研究旨在探讨 circ_0063804 对卵巢癌（OC）发展和顺铂耐药性的影响，为 OC 治疗提供新靶点。
本研究纳入了 108 名 OC 患者。通过 RNase R 检测 circ_0063804 的环状结构。通过转染上调或下调 OC 细胞中的 circ_0063804 表达。用细胞计数试剂盒-8 法和集落形成实验评估细胞增殖。用流式细胞术检测细胞凋亡。通过 MTT 实验探讨 OC 细胞对顺铂的耐药性。通过荧光素酶报告实验进行研究。用 qRT-PCR 和 Western blot 检测基因表达。利用裸鼠进行异种移植肿瘤实验。通过免疫组化检测异种移植肿瘤中 Ki67 的表达。
OC 患者中 circ_0063804 表达上调，且预示着不良预后（<0.05）。circ_0063804 具有稳定的环状结构。circ_0063804 增强了 OC 细胞的增殖、顺铂耐药性并降低了细胞凋亡（<0.01）。OC 患者中 miR-1276 表达下调，并与 circ_0063804 结合。CLU 直接受到 miR-1276 的抑制，且在 OC 患者中上调。circ_0063804 通过海绵吸附 miR-1276 增强 CLU 表达，从而加剧 OC 细胞的恶性表型和对顺铂的耐药性（<0.01）。沉默 circ_0063804 抑制 OC 细胞生长、顺铂耐药性和 Ki67 表达（<0.01）。
circ_0063804 通过海绵吸附 miR-1276 增强 CLU 表达，促进 OC 细胞增殖和对顺铂的耐药性。
