Zhang Hua, Huang Haitao, Xu Xiaomei, Wang Haiying, Wang Jianxiang, Yao Zuoyi, Xu Xiaoyan, Wu Qian, Xu Fenlan
1Department of Gastroenterology, The Fifth People's Hospital of Chengdu, No. 33 Mashi Street, Wenjiang District, Chengdu, 611130 China.
2Department of Respiratory, The Fifth People's Hospital of Chengdu, No. 33 Mashi Street, Wenjiang District, Chengdu, 611130 China.
Cancer Cell Int. 2019 Dec 26;19:350. doi: 10.1186/s12935-019-1046-0. eCollection 2019.
Gastric cancer (GC) is one common cancer which occurs in the stomach leading to high mortality around the world. Long non-coding RNAs (lncRNAs) were found overexpressed or silenced in the occurrence and progression of multiple cancers including GC.
The gene expression level in GC tissues and cells were analyzed by RT-qPCR. CCK-8, colony formation, flow cytometry and transwell assays were performed for the function analysis of HLA complex group 11 (HCG11). The mechanism study for HCG11 was conducted using RIP, RNA pull down and luciferase reporter assays.
HCG11 was discovered highly expressed in GC tissues and cells. Depletion experiments were used to evaluate HCG11 silence on cell proliferation, migration and apoptosis. Moreover, Wnt signaling pathway was found as a tumor promoter in GC. RIP assay, RNA pull down assay and luciferase reporter assay were performed to illustrate the relationship of HCG11, miR-1276 and CTNNB1. Rescue assays revealed that HCG11/miR-1276/CTNNB1 axis regulated the incidence and development of GC. Tumor formation in mice proved that HCG11 was negatively correlated with miR-1276 and had positively correlation with CTNNB1.
Overall, HCG11 accelerated proliferation and migration in GC through miR-1276/CTNNB1 and Wnt signaling pathway, revealing that HCG11 could be a brand new target for GC.
胃癌（GC）是一种常见的发生于胃部的癌症，在全球导致高死亡率。长链非编码RNA（lncRNAs）在包括胃癌在内的多种癌症的发生和发展过程中被发现存在过表达或沉默现象。
通过RT-qPCR分析胃癌组织和细胞中的基因表达水平。采用CCK-8、集落形成、流式细胞术和Transwell实验对HLA复合体组11（HCG11）进行功能分析。使用RIP、RNA下拉和荧光素酶报告基因实验对HCG11进行机制研究。
发现HCG11在胃癌组织和细胞中高表达。采用敲减实验评估HCG11沉默对细胞增殖、迁移和凋亡的影响。此外，发现Wnt信号通路在胃癌中作为肿瘤促进因子。进行RIP实验、RNA下拉实验和荧光素酶报告基因实验以阐明HCG11、miR-1276和CTNNB1之间的关系。挽救实验表明HCG11/miR-1276/CTNNB1轴调节胃癌的发生和发展。小鼠肿瘤形成实验证明HCG11与miR-1276呈负相关，与CTNNB1呈正相关。
总体而言，HCG11通过miR-1276/CTNNB1和Wnt信号通路加速胃癌细胞的增殖和迁移，表明HCG11可能是胃癌一个全新的治疗靶点。
