• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

槲皮素治疗可保护高血糖诱导的大鼠跟腱免受氧化应激。

Quercetin treatment protects the Achilles tendons of rats from oxidative stress induced by hyperglycemia.

机构信息

Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Hyogo, 650-0017, Japan.

出版信息

BMC Musculoskelet Disord. 2022 Jun 10;23(1):563. doi: 10.1186/s12891-022-05513-4.

DOI:10.1186/s12891-022-05513-4
PMID:35689230
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9188208/
Abstract

BACKGROUND

Quercetin, a flavonoid abundantly in vegetables and fruits, exerts antioxidant and anti-inflammatory effects. We investigated the protective effects of quercetin against oxidative stress in the Achilles tendons of diabetic rats.

METHODS

Cells were collected from the Achilles tendons of Sprague-Dawley rats and cultured under four conditions: regular glucose (RG) without quercetin (Quer-), RG with quercetin (Quer +), high-glucose (HG) Quer-, and HG Quer + . The expression of genes related to NADPH oxidase (NOX) and inflammation, reactive oxygen species accumulation, and apoptosis rates was analyzed. Additionally, diabetic rats were divided into two groups and subjected to quercetin (group Q) or no quercetin (group C) treatment. Histological evaluation and expression analysis of relevant genes in the Achilles tendon were performed.

RESULTS

In rat tendon-derived cells, the expression of Nox1, Nox4, and Il6; reactive oxygen species accumulation; and apoptosis rates were significantly decreased by quercetin treatment in the HG group. The collagen fiber arrangement was significantly disorganized in the diabetic rat Achilles tendons in group C compared with that in group Q. The mRNA and protein expression levels of NOX1 and NOX4 were significantly decreased upon quercetin treatment. Furthermore, the expression of Il6, type III collagen, Mmp2, and Timp2 was significantly decreased, whereas that of type I collagen was significantly increased in group Q compared with that in group C.

CONCLUSIONS

Quercetin treatment decreases NOX expression and thus exerts antioxidant and anti-inflammatory effects in the Achilles tendons of diabetic rats. Quercetin treatment may be effective against diabetic tendinopathy.

摘要

背景

槲皮素是一种在蔬菜和水果中含量丰富的类黄酮，具有抗氧化和抗炎作用。我们研究了槲皮素对糖尿病大鼠跟腱氧化应激的保护作用。

方法

从 Sprague-Dawley 大鼠的跟腱中收集细胞，并在四种条件下培养：无槲皮素的常规葡萄糖（RG）（Quer-）、有槲皮素的 RG（Quer+）、高葡萄糖（HG） Quer-和 HG Quer+。分析与 NADPH 氧化酶（NOX）和炎症、活性氧积累和细胞凋亡率相关的基因表达。此外，将糖尿病大鼠分为两组，分别给予槲皮素（Q 组）或无槲皮素（C 组）治疗。对跟腱的组织学评价和相关基因的表达分析。

结果

在大鼠肌腱源性细胞中，HG 组中槲皮素处理可显著降低 Nox1、Nox4 和 Il6 的表达、活性氧积累和细胞凋亡率。与 Q 组相比，C 组糖尿病大鼠跟腱中的胶原纤维排列明显紊乱。Q 组中 NOX1 和 NOX4 的 mRNA 和蛋白表达水平明显降低。此外，与 C 组相比，Q 组中 Il6、III 型胶原、Mmp2 和 Timp2 的表达明显降低，而 I 型胶原的表达明显增加。

结论

槲皮素治疗可降低 NOX 的表达，从而发挥抗氧化和抗炎作用，改善糖尿病大鼠的跟腱病变。槲皮素治疗可能对糖尿病性肌腱病有效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/d4c5254f87b4/12891_2022_5513_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/d0c61e730b13/12891_2022_5513_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/d855eb993040/12891_2022_5513_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/7ce220649cf2/12891_2022_5513_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/fbc5ee340a4e/12891_2022_5513_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/6029745ba684/12891_2022_5513_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/cd2d8872efba/12891_2022_5513_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/c74bfb790983/12891_2022_5513_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/d4c5254f87b4/12891_2022_5513_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/d0c61e730b13/12891_2022_5513_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/d855eb993040/12891_2022_5513_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/7ce220649cf2/12891_2022_5513_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/fbc5ee340a4e/12891_2022_5513_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/6029745ba684/12891_2022_5513_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/cd2d8872efba/12891_2022_5513_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/c74bfb790983/12891_2022_5513_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a774/9188208/d4c5254f87b4/12891_2022_5513_Fig8_HTML.jpg

相似文献

1
Quercetin treatment protects the Achilles tendons of rats from oxidative stress induced by hyperglycemia.槲皮素治疗可保护高血糖诱导的大鼠跟腱免受氧化应激。
BMC Musculoskelet Disord. 2022 Jun 10;23(1):563. doi: 10.1186/s12891-022-05513-4.
2
In vitro and in vivo tenocyte-protective effectiveness of dehydroepiandrosterone against high glucose-induced oxidative stress.去氢表雄酮对高糖诱导的氧化应激的肌腱细胞保护作用的体内外研究。
BMC Musculoskelet Disord. 2021 Jun 5;22(1):519. doi: 10.1186/s12891-021-04398-z.
3
Antioxidant effect of nicotinamide mononucleotide in tendinopathy.烟酰胺单核苷酸对腱病的抗氧化作用。
BMC Musculoskelet Disord. 2022 Mar 14;23(1):249. doi: 10.1186/s12891-022-05205-z.
4
Exogenous Hydrogen Sulfide Attenuates Cardiac Fibrosis Through Reactive Oxygen Species Signal Pathways in Experimental Diabetes Mellitus Models.外源性硫化氢通过活性氧信号通路减轻实验性糖尿病模型中的心脏纤维化
Cell Physiol Biochem. 2015;36(3):917-29. doi: 10.1159/000430266. Epub 2015 Jun 12.
5
Evaluation of Therapeutic Effects of Quercetin Against Achilles Tendinopathy in Rats via Oxidative Stress, Inflammation, Apoptosis, Autophagy, and Metalloproteinases.槲皮素通过氧化应激、炎症、细胞凋亡、自噬和金属蛋白酶对大鼠跟腱病治疗效果的评价。
Am J Sports Med. 2022 Feb;50(2):486-498. doi: 10.1177/03635465211059821. Epub 2021 Dec 15.
6
Nox4-derived reactive oxygen species mediate cardiomyocyte injury in early type 1 diabetes.Nox4 产生的活性氧介导早期 1 型糖尿病中的心肌细胞损伤。
Am J Physiol Cell Physiol. 2012 Feb 1;302(3):C597-604. doi: 10.1152/ajpcell.00331.2011. Epub 2011 Oct 26.
7
The effects of high glucose condition on rat tenocytes and rat Achilles tendon .高糖环境对大鼠肌腱细胞和大鼠跟腱的影响。
Bone Joint Res. 2018 Jun 5;7(5):362-372. doi: 10.1302/2046-3758.75.BJR-2017-0126.R2. eCollection 2018 May.
8
Quercetin inhibited epithelial mesenchymal transition in diabetic rats, high-glucose-cultured lens, and SRA01/04 cells through transforming growth factor-β2/phosphoinositide 3-kinase/Akt pathway.槲皮素通过转化生长因子-β2/磷酸肌醇3-激酶/蛋白激酶B通路抑制糖尿病大鼠、高糖培养的晶状体及SRA01/04细胞中的上皮-间质转化。
Mol Cell Endocrinol. 2017 Sep 5;452:44-56. doi: 10.1016/j.mce.2017.05.011. Epub 2017 May 10.
9
Experimental diabetes induces structural, inflammatory and vascular changes of Achilles tendons.实验性糖尿病可引起跟腱的结构、炎症和血管变化。
PLoS One. 2013 Oct 9;8(10):e74942. doi: 10.1371/journal.pone.0074942. eCollection 2013.
10
Antioxidant treatment with quercetin ameliorates erectile dysfunction in streptozotocin-induced diabetic rats.槲皮素抗氧化治疗可改善链脲佐菌素诱导的糖尿病大鼠的勃起功能障碍。
J Biosci Bioeng. 2011 Sep;112(3):215-8. doi: 10.1016/j.jbiosc.2011.05.013. Epub 2011 Jun 14.

引用本文的文献

1
Quercetin in Idiopathic Pulmonary Fibrosis and Its Comorbidities: Gene Regulatory Mechanisms and Therapeutic Implications.槲皮素在特发性肺纤维化及其合并症中的作用：基因调控机制与治疗意义
Genes (Basel). 2025 Jul 23;16(8):856. doi: 10.3390/genes16080856.
2
Contribution and Regulation of HIF-1α in Testicular Injury Induced by Diabetes Mellitus.缺氧诱导因子-1α在糖尿病所致睾丸损伤中的作用及调控
Biomolecules. 2025 Aug 19;15(8):1190. doi: 10.3390/biom15081190.
3
Investigation of the molecular mechanism of Xiaoluo Wan in thyroid-associated ophthalmopathy: network analysis and in vivo study.

本文引用的文献

1
Evaluation of apocynin in vitro on high glucose-induced oxidative stress on tenocytes.体外评估白杨素对高糖诱导的腱细胞氧化应激的影响。
Bone Joint Res. 2020 May 16;9(1):23-28. doi: 10.1302/2046-3758.991.BJR-2019-0074.R1. eCollection 2020 Jan.
2
Quercetin nanoparticles with enhanced bioavailability as multifunctional agents toward amyloid induced neurotoxicity.具有增强生物利用度的槲皮素纳米颗粒作为针对淀粉样蛋白诱导的神经毒性的多功能剂。
J Mater Chem B. 2018 Mar 7;6(9):1387-1393. doi: 10.1039/c7tb03053c. Epub 2018 Feb 19.
3
Reactive oxygen species (ROS) as pleiotropic physiological signalling agents.
消瘰丸治疗甲状腺相关性眼病的分子机制研究：网络分析及体内研究
Hereditas. 2025 Jul 22;162(1):137. doi: 10.1186/s41065-025-00499-0.
4
Epigallocatechin-3-Gallate (EGCG)-Loaded Hyaluronic Acid Hydrogel Seems to Be Effective in a Rat Model of Collagenase-Induced Achilles Tendinopathy.负载表没食子儿茶素-3-没食子酸酯（EGCG）的透明质酸水凝胶在胶原酶诱导的大鼠跟腱病模型中似乎有效。
J Funct Biomater. 2025 Feb 10;16(2):55. doi: 10.3390/jfb16020055.
5
Does quercetin affect tendon healing? An experimental study in a rat model of Achilles tendon injury.槲皮素会影响肌腱愈合吗？一项针对跟腱损伤大鼠模型的实验研究。
Front Med (Lausanne). 2025 Feb 5;12:1522517. doi: 10.3389/fmed.2025.1522517. eCollection 2025.
6
Therapeutic Efficacy of Medicinal Plants with Allopathic Medicine in Musculoskeletal Diseases.药用植物与对抗疗法药物在肌肉骨骼疾病中的治疗效果
Int J Plant Anim Environ Sci. 2024;14(4):104-129. doi: 10.26502/ijpaes.4490170. Epub 2024 Dec 23.
7
Therapeutic Potential of Quercetin as an Antioxidant for Bone-Muscle-Tendon Regeneration and Aging.槲皮素作为抗氧化剂在骨-肌肉-肌腱再生与衰老方面的治疗潜力
Aging Dis. 2024 Jun 24;16(3):1414-1437. doi: 10.14336/AD.2024.0282.
8
Role of oxidative stress in the concurrent development of osteoporosis and tendinopathy: Emerging challenges and prospects for treatment modalities.氧化应激在骨质疏松症和腱病并发发展中的作用：治疗方式的新挑战和前景。
J Cell Mol Med. 2024 Jul;28(13):e18508. doi: 10.1111/jcmm.18508.
9
Current research trends on the effect of diabetes mellitus on rotator cuff tendon healing/tendinopathy.目前关于糖尿病对肩袖肌腱愈合/肌腱病影响的研究趋势。
Arch Orthop Trauma Surg. 2024 Jun;144(6):2491-2500. doi: 10.1007/s00402-024-05350-1. Epub 2024 May 2.
10
Hypoxia-Inducible Factor and Oxidative Stress in Tendon Degeneration: A Molecular Perspective.肌腱退变中的缺氧诱导因子与氧化应激：分子视角
Antioxidants (Basel). 2024 Jan 10;13(1):86. doi: 10.3390/antiox13010086.
活性氧（ROS）作为多效生理信号剂。
Nat Rev Mol Cell Biol. 2020 Jul;21(7):363-383. doi: 10.1038/s41580-020-0230-3. Epub 2020 Mar 30.
4
Antioxidant Activities of Quercetin and Its Complexes for Medicinal Application.槲皮素及其配合物的药用抗氧化活性。
Molecules. 2019 Mar 21;24(6):1123. doi: 10.3390/molecules24061123.
5
The natural phenolic compounds as modulators of NADPH oxidases in hypertension.天然酚类化合物作为高血压中 NADPH 氧化酶的调节剂。
Phytomedicine. 2019 Mar 1;55:200-213. doi: 10.1016/j.phymed.2018.08.002. Epub 2018 Aug 6.
6
Quercetin alleviates high glucose-induced damage on human umbilical vein endothelial cells by promoting autophagy.槲皮素通过促进自噬缓解高糖诱导的人脐静脉内皮细胞损伤。
Phytomedicine. 2019 Mar 15;56:183-193. doi: 10.1016/j.phymed.2018.11.008. Epub 2018 Nov 8.
7
Flavonoid compounds and antibacterial mechanisms of different parts of white guava ( L. cv. Pearl).白番石榴（L. cv. Pearl）不同部位的类黄酮化合物和抗菌机制。
Nat Prod Res. 2020 Jun;34(11):1621-1625. doi: 10.1080/14786419.2018.1522313. Epub 2018 Oct 18.
8
The effects of high glucose condition on rat tenocytes and rat Achilles tendon .高糖环境对大鼠肌腱细胞和大鼠跟腱的影响。
Bone Joint Res. 2018 Jun 5;7(5):362-372. doi: 10.1302/2046-3758.75.BJR-2017-0126.R2. eCollection 2018 May.
9
Formulation and evaluation of mixed polymeric micelles of quercetin for treatment of breast, ovarian, and multidrug resistant cancers.槲皮素混合聚合物胶束的制剂与评价及其在乳腺癌、卵巢癌和多药耐药性癌症治疗中的应用。
Int J Nanomedicine. 2018 May 16;13:2869-2881. doi: 10.2147/IJN.S153094. eCollection 2018.
10
Quercetin conjugated with silica nanoparticles inhibits tumor growth in MCF-7 breast cancer cell lines.槲皮素与硅纳米粒子结合可抑制 MCF-7 乳腺癌细胞系的肿瘤生长。
Biochem Biophys Res Commun. 2018 Jun 12;500(4):860-865. doi: 10.1016/j.bbrc.2018.04.174. Epub 2018 Apr 26.