Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, China; Guangdong Key Laboratory for Dental Disease Prevention and Control, Sun Yat-Sen University, Guangzhou, China.
Arch Oral Biol. 2022 Sep;141:105471. doi: 10.1016/j.archoralbio.2022.105471. Epub 2022 Jun 6.
We aimed to investigate the composition and functions discrepancy of supragingival plaque associated with active deciduous teeth caries in mixed dentitions.
Thirty-three subjects with mixed dentition participated in this study. Children with deciduous teeth caries (dt ≥ 3) were recruited to the caries group, whereas children without deciduous teeth caries (dt = 0) were recruited to the caries-free group. Plaque were collected from deciduous teeth surface and permanent teeth surface respectively. A total of 66 samples of dental plaque were collected and conserved. Illumina 16S rRNA sequencing and diversity analysis were performed for microbiome. Untargeted liquid chromatograph-mass (LC-MS) and partial least squares discriminant analysis were performed for metabolome.
A dominant microbiome of 8 phyla and 22 genera were detected. The alpha diversity indices did not detect differences between the caries and caries-free groups (p > 0.05). Beta diversity analysis showed that the microbiota composition was similar between subgroups. Comparative analysis at genus level did not detect difference between caries and caries-free subgroups. The metabolomics analysis yielded 419 biochemical metabolites, 56 of which were related to caries status. Metabolites in glucose metabolism and byproducts of oxidative stress were identified as related to dental caries in mixed dentition. Dominant bacteria are positively correlated with metabolites, such as Streptococcus and organic acids.
The upgrade of glucose metabolism and oxidative stress was observed in caries status. Functions discrepancy of oral microbiome may be more pronounced than the composition of oral microbiome with active dental caries in mixed dentitions.
本研究旨在探究混合牙列中活跃性乳牙龋齿相关的龈上菌斑的组成和功能差异。
本研究纳入了 33 名混合牙列的受试者。患有乳牙龋齿（dt≥3）的儿童被招募至龋齿组，而无乳牙龋齿（dt=0）的儿童被招募至无龋齿组。分别从乳牙和恒牙表面采集菌斑。共采集并保存了 66 份牙菌斑样本。对微生物组进行 Illumina 16S rRNA 测序和多样性分析，对代谢组进行非靶向液相色谱-质谱（LC-MS）和偏最小二乘判别分析。
检测到了 8 个门和 22 个属的主要微生物群。α多样性指数在龋齿组和无龋齿组之间未检测到差异（p＞0.05）。β多样性分析显示，亚组间的微生物群落组成相似。在属水平的比较分析中，龋齿组和无龋齿组之间未检测到差异。代谢组学分析得到了 419 种生化代谢物，其中 56 种与龋齿状况相关。鉴定出与混合牙列龋齿相关的代谢物为葡萄糖代谢和氧化应激的副产物。优势细菌与代谢物呈正相关，如链球菌和有机酸。
在龋齿状态下观察到葡萄糖代谢和氧化应激的升级。与活跃性乳牙龋齿相关的口腔微生物组的功能差异可能比其组成更为显著。
