Laboratory of Lingnan Modern Agriculture, Guangdong Province Key Laboratory of Animal Nutritional Regulation, College of Animal Science, South China Agricultural University, Guangzhou China.
Anhui maitebao Bioengineering Co., Ltd, Hefei, China.
Poult Sci. 2022 Aug;101(8):101912. doi: 10.1016/j.psj.2022.101912. Epub 2022 Apr 8.
The beneficial action of probiotics is questioned time and again due to the loss of their survivability under gastrointestinal conditions, particularly gastric acid. In this experiment, a probiotic species was encapsulated to improve its delivery to the distal parts, and its effects on production performance, gut health, and microbial profile in broilers were investigated. A total of 240 Arbor acres (AA) broilers were randomly allotted into 3 treatments with 8 replicate pens per treatment and 10 broilers in each pen for 42 d. Dietary treatments were 1) basal feed without any additives (CON), 2) CON+15 ppm Virginiamycin (ANT), and 3) CON+500 ppm encapsulated Lactobacillus paracaesi (ELP). The result showed that the addition of ELP to the feed did not affect growth performance and carcass characteristics significantly. However, ELP increased the ratio of villus height to crypt depth (P < 0.05) and mRNA expression of ZO-1 (P < 0.05) relative to the CON or ANT group. Similarly, qPCR showed that dietary supplementation of ELP raised gene expression of the anti-inflammatory cytokine and tended to decrease proinflammatory cytokines resulting improve in immunity. Moreover, chicks fed with ELP had lower malondialdehyde (MDA) (P < 0.05) than CON and lower reactive oxygen species (ROS) (P < 0.05) level than ANT in serum. In contrast, the total antioxidant capacity (TAOC) level was tended to increase. The ammonia level of ileum and cecum chyme was decreased (P < 0.05) in the ELP group than CON while the level of propionic acid of cecal content was increased (P < 0.05). 16S rRNA sequencing revealed the dietary treatment modulated the diversity and composition of cecal microflora. At the phylum level, Bacteroidetes was enriched, and Proteobacteria was depleted in the ELP group. At the genus level, ELP increased Bacteroides (P < 0.05) compared to control. The results indicate that oral delivery of probiotics via microcapsule could impart beneficial effects on birds and be used as an alternative to antibiotics.
由于益生菌在胃肠道条件下，尤其是胃酸环境下的生存能力丧失，它们的有益作用一直受到质疑。在本实验中，我们对一种益生菌进行了包埋处理，以改善其向远端的传递，并研究了其对肉鸡生产性能、肠道健康和微生物谱的影响。将 240 只 Arbor acres（AA）肉鸡随机分为 3 个处理组，每个处理组设 8 个重复笼，每个笼 10 只鸡，试验期为 42d。日粮处理为 1）基础日粮，不添加任何添加剂（CON）；2）CON+15ppm 维吉尼亚霉素（ANT）；3）CON+500ppm 包被的副干酪乳杆菌（ELP）。结果表明，在饲料中添加 ELP 对生长性能和胴体特性没有显著影响。然而，ELP 增加了绒毛高度与隐窝深度的比值（P＜0.05）和 ZO-1 的 mRNA 表达（P＜0.05），与 CON 或 ANT 组相比。同样，qPCR 显示，日粮中添加 ELP 提高了抗炎细胞因子的基因表达，且有降低促炎细胞因子的趋势，从而改善了免疫力。此外，与 CON 和 ANT 相比，饲喂 ELP 的鸡血清中的丙二醛（MDA）（P＜0.05）和活性氧（ROS）（P＜0.05）水平降低，而总抗氧化能力（TAOC）水平有升高的趋势。ELP 组回肠和盲肠内容物的氨水平降低（P＜0.05），而盲肠内容物丙酸水平升高（P＜0.05）。16S rRNA 测序显示，日粮处理调节了盲肠微生物群落的多样性和组成。在门水平上，拟杆菌门增多，变形菌门减少。在属水平上，与对照组相比，ELP 增加了拟杆菌属（P＜0.05）。结果表明，通过微胶囊口服递送益生菌可以对禽类产生有益的影响，并可作为抗生素的替代品。
