Deoxynivalenol (DON) and Zearalenone (ZEN), common symbiotic mycotoxins found in mold-contaminated cereal feed, adversely affect broiler' health. has various pharmacological effects including antibacterial, antioxidant and immunomodulatory. This study aimed to investigate the effects of the long-term intake of low doses of DON and ZEN on growth performance and intestinal health of broilers, as well as the potential protective effect of supplementary extract (GUE) in an 84-day feeding experiment. A total of 315 one-day-old male broilers were randomly assigned to three treatments: basal diet (CON), MOL diet (where 5% of corn in the basal diet was replaced with an equal amount of naturally moldy corn) containing DON and ZEN at 1.25 and 1.29 mg/kg, and MGUE diet supplemented with 0.1% GUE in the MOL diet. The MOL diet reduced the body weight (BW) of broilers at 56 and 84 day, body weight gain (BWG) and feed intake (FI) aged 1-56 and 1-84 days, and the feed conversion ratio (FCR) aged 1-84 days, as well as villus height (VH) and the villus/crypt (V/C) ratio, SOD and GSH-Px activities, and the expression of , and , while increasing MDA level, the expression of , and in the jejunum of broilers. Additionally, MOL diet decreased the to (F/B) ratio and abundances of ( and ), and , while increasing ( and ), (), and () in the ceca. In contrast, MGUE diet improved growth performance and returned it to a level comparable to that of the CON diet, increased VH and V/C ratio, SOD and GSH-Px activity, , and expression, while reducing MDA level, the expression of , and in the jejunum. Moreover, MGUE diet had a greater F/B ratio and abundance of ( and ) and , while reducing ( and ), () and () in cecum. In conclusion, the long-term consumption of a low-dose DON-ZEN contaminated diet decreases growth performance and disrupts intestinal health and microbiota balance in broilers; however, dietary supplementation with GUE effectively mitigates the damage caused by DON-ZEN contamination.