Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China.
Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China.
Poult Sci. 2022 Aug;101(8):101936. doi: 10.1016/j.psj.2022.101936. Epub 2022 Apr 30.
Two experiments were carried out to study the effect of in ovo manganese (Mn) injection on the embryonic development, antioxidation, hatchability, and performances of offspring broilers under normal temperature (NT) and high temperature (HT). Experiment 1 was conducted to investigate the effect of in ovo Mn injection on the embryonic hatchability of Arbor Acres broiler breeders. On D 9 of incubation, a total of 684 fertilized eggs were randomly allocated to 6 treatments: the non-injected positive control (niPC) and treatments injected with 0 (the negative control, iNC), 6.25, 12.5, 25.0, or 50.0 μg Mn/egg as Mn sulfate. Experiment 2 was conducted to investigate the effect of in ovo Mn injection on the embryonic development, antioxidation and performance of offspring broilers under NT and HT. A total of 792 fertilized eggs were randomly allocated to 6 treatments in a 1 (niPC) + 1 (iNC) + 2 (injected Mn sources: Mn sulfate and Mn proteinate) × 2 (injected Mn levels: 12.5 and 25.0 μg/egg) factorial arrangement during the embryonic stage and D1 to 28 at NT. Then, 288 birds were allotted to 12 treatments in a 6 (the above embryonic treatments) × 2 (environmental temperatures: NT-22℃ vs HT-34℃) factorial arrangement from D 29 to 42. The results showed that Mn injection affected (P < 0.03) the hatchability and the maximum level of in ovo injected Mn was 25.0 μg Mn/egg. The Mn injection upregulated (P < 0.05) Mn-containing superoxide dismutase mRNA expression in the embryonic heart compared to the iNC. Hyperthermia decreased (P < 0.05) ADG and ADFI, breast muscle percentage, plsma alkaline phosphatase activity, and red color values of breast and thigh muscles, but increased (P < 0.05) F/G, plasma aspartate aminotransferase and lactic dehydrogenase activities, total cholesterol, uric acid and triiodothyronine contents, abdominal fat, light values of breast and thigh muscles of offspring broilers. The results suggest that in ovo Mn injection can enhance antioxidant ability in the chick embryonic heart.
进行了两项实验，以研究鸡胚内注射锰（Mn）对正常温度（NT）和高温（HT）下雏鸡胚胎发育、抗氧化、孵化率和后代肉鸡性能的影响。实验 1 旨在研究鸡胚内注射 Mn 对阿伯丁·阿可斯肉鸡种鸡胚胎孵化率的影响。孵化第 9 天，将 684 枚受精蛋随机分配到 6 个处理组：非注射阳性对照组（niPC）和注射 0（阴性对照，iNC）、6.25、12.5、25.0 或 50.0μg Mn/枚蛋的 Mn 硫酸盐。实验 2 旨在研究鸡胚内注射 Mn 对 NT 和 HT 下雏鸡胚胎发育、抗氧化和性能的影响。在胚胎期，将 792 枚受精蛋随机分配到 1（niPC）+1（iNC）+2（注射 Mn 源：Mn 硫酸盐和 Mn 蛋白盐）×2（注射 Mn 水平：12.5 和 25.0μg/枚蛋）因子设计中，然后在 NT 下从 D1 到 28 天进行。然后，将 288 只鸡分配到 12 个处理组，即 6（上述胚胎处理）×2（环境温度：NT-22℃与 HT-34℃）因子设计中，从 D29 到 42 天进行。结果表明，Mn 注射影响（P<0.03）孵化率，最大鸡胚内注射 Mn 水平为 25.0μg Mn/枚蛋。与 iNC 相比，Mn 注射增加（P<0.05）了胚胎心脏中含 Mn 的超氧化物歧化酶 mRNA 的表达。高温降低（P<0.05）了 ADG 和 ADFI、胸肌百分比、血浆碱性磷酸酶活性和胸肌和腿肌的红色值，但增加了（P<0.05）F/G、血浆天冬氨酸氨基转移酶和乳酸脱氢酶活性、总胆固醇、尿酸和三碘甲状腺原氨酸含量、腹部脂肪、胸肌和腿肌的光值。结果表明，鸡胚内注射 Mn 可以增强雏鸡心脏的抗氧化能力。
