School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
Institute of Biosciences, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
Sci Rep. 2024 Feb 28;14(1):4883. doi: 10.1038/s41598-024-55478-8.
Supplementing minerals beyond dietary requirements can increase the risk of toxicity and mineral excretion, making the selection of more bioavailable sources crucial. Thus, this work aimed to use metalloproteomics tools to investigate possible alterations in the hepatic proteome of broilers fed with diets containing two sources (sulfate and hydroxychloride) and two levels of copper (15 and 150 ppm) and manganese (80 and 120 ppm), totaling four treatments: low Cu/Mn SO, high Cu/Mn SO, low Cu/Mn (OH)Cl and high Cu/Mn (OH)Cl. The difference in abundance of protein spots and copper and manganese concentrations in liver and protein pellets were analyzed by analysis of variance with significance level of 5%. The Cu and Mn concentrations determined in liver and protein pellets suggested greater bioavailability of hydroxychloride sources. We identified 19 Cu-associated proteins spots, 10 Mn-associated protein spots, and 5 Cu and/or Mn-associated protein spots simultaneously. The analysis also indicated the induction of heat shock proteins and detoxification proteins in broilers fed with high levels of copper and manganese, suggesting the involvement of these proteins in metal tolerance and stress.
补充超出膳食需求的矿物质可能会增加毒性和矿物质排泄的风险，因此选择更具生物利用度的来源至关重要。因此，本工作旨在使用金属蛋白质组学工具研究饲粮中添加两种铜（15 和 150 ppm）和锰（80 和 120 ppm）来源（硫酸盐和羟氯酸盐）的肉鸡肝脏蛋白质组中可能发生的变化，共设置四个处理组：低 Cu/Mn SO4、高 Cu/Mn SO4、低 Cu/Mn（OH）Cl 和高 Cu/Mn（OH）Cl。通过方差分析（显著性水平为 5%）对蛋白点丰度差异、肝脏和蛋白沉淀中的铜和锰浓度进行分析。肝脏和蛋白沉淀中的 Cu 和 Mn 浓度表明羟氯酸盐来源的生物利用度更高。我们鉴定到 19 个与 Cu 相关的蛋白点、10 个与 Mn 相关的蛋白点和 5 个同时与 Cu 和/或 Mn 相关的蛋白点。分析还表明，高铜和高锰饲粮可诱导肉鸡的热休克蛋白和解毒蛋白，表明这些蛋白参与金属耐受和应激反应。
