College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610000, Sichuan, China; Department of Human Nutrition, Food and Animal Sciences, University of Hawaii at Manoa, Honolulu, HI, United States.
Department of Human Nutrition, Food and Animal Sciences, University of Hawaii at Manoa, Honolulu, HI, United States.
J Proteomics. 2022 Aug 15;265:104650. doi: 10.1016/j.jprot.2022.104650. Epub 2022 Jun 8.
Adipose tissue not only affects meat quality and animal productivity, but also participates in inflammation and immunity. Ningxiang pig is famous for their excellent meat quality, disease resistance and tolerance of roughage. It is not yet well known how proteins in adipose tissue is dynamically regulated during the growth of Ningxiang pig. This report studies the proteomic changes in subcutaneous adipose tissue in Ningxiang pigs to gain a better understanding of the molecular mechanism of fat development during the growth period. By TMT-labeled quantitative proteomic analysis of subcutaneous adipose tissue of 9 purebred Ningxiang pigs of different ages, we identified 2533 unique proteins and 716 differentially abundant proteins (DAPs), of which more than half of the DAPs were concentrated in the 90d-210d period. Retrograde endocannabinoid signaling was only significantly enriched in DAPs of N90d vs N30d, Alcoholism and Graft-versus-host disease were only significantly enriched in DAPs of N210d vs N90d. Proteins related to dilated cardiomyopathy was found to be an important pathway in fat development and lipid metabolism. A variety of novel DAPs involved in maintaining mitochondrial function and cell viability, such as NDUFS6, SDHB, COX5A, ATP5D and TNNT1, which play a role in controlling the prediction networks, may indirectly regulate the development and functional maintenance of adipocytes. SIGNIFICANCE: These age-dependent DAPs discovered in this study may help expand the understanding of the molecular mechanisms of the development, function maintenance and transformation of adipose tissue in Ningxiang pig for developing new strategies for improving meat quality and pig breeding in the future.
脂肪组织不仅影响肉质和动物生产性能，而且参与炎症和免疫反应。宁乡猪以其优良的肉质、抗病力和粗饲耐受性而闻名。目前尚不清楚宁乡猪在生长过程中脂肪组织中的蛋白质是如何动态调节的。本研究通过对不同年龄的 9 头宁乡猪皮下脂肪组织的 TMT 标记定量蛋白质组学分析，研究了宁乡猪皮下脂肪组织的蛋白质组变化，以期更好地了解脂肪发育的分子机制。我们共鉴定到 2533 个独特蛋白和 716 个差异丰度蛋白（DAP），其中超过一半的 DAP 集中在 90d-210d 期间。逆行内源性大麻素信号通路仅在 N90d 与 N30d 的 DAP 中显著富集，酒精中毒和移植物抗宿主病仅在 N210d 与 N90d 的 DAP 中显著富集。扩张型心肌病相关蛋白被发现是脂肪发育和脂质代谢的重要途径。许多与维持线粒体功能和细胞活力相关的新型 DAPs，如 NDUFS6、SDHB、COX5A、ATP5D 和 TNNT1，在控制预测网络中发挥作用，可能间接调节脂肪细胞的发育和功能维持。这些年龄依赖性 DAPs 的发现可能有助于扩大对宁乡猪脂肪组织发育、功能维持和转化的分子机制的理解，为未来改善肉质和猪种培育开发新策略提供参考。
