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新型 X 射线/可见显微镜 MAXWELL 技术,可实现各向同性分辨率的快速三维纳米成像。

The new X-ray/visible microscopy MAXWELL technique for fast three-dimensional nanoimaging with isotropic resolution.

机构信息

RIKEN/SPring-8 Center, Hyogo, 679-5148, Japan.

Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.

出版信息

Sci Rep. 2022 Jun 11;12(1):9668. doi: 10.1038/s41598-022-13377-w.

DOI:10.1038/s41598-022-13377-w
PMID:35690597
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9188605/
Abstract

Microscopy by Achromatic X-rays With Emission of Laminar Light (MAXWELL) is a new X-ray/visible technique with attractive characteristics including isotropic resolution in all directions, large-volume imaging and high throughput. An ultrathin, laminar X-ray beam produced by a Wolter type I mirror irradiates the sample stimulating the emission of visible light by scintillating nanoparticles, captured by an optical system. Three-dimensional (3D) images are obtained by scanning the specimen with respect to the laminar beam. We implemented and tested the technique with a high-brightness undulator at SPring-8, demonstrating its validity for a variety of specimens. This work was performed under the Synchrotrons for Neuroscience-an Asia-Pacific Strategic Enterprise (SYNAPSE) collaboration.

摘要

利用发射层状光的消色差 X 射线显微镜(MAXWELL)是一种新的 X 射线/可见光技术,具有各向同性分辨率、大容量成像和高通量等吸引人的特点。由 Wolter 型 I 镜产生的超薄层状 X 射线束照射样品,刺激闪烁纳米粒子发射可见光,这些可见光由光学系统捕获。通过相对于层状光束扫描样品获得三维(3D)图像。我们在 SPring-8 上实现并测试了该技术,证明了它对各种样品的有效性。这项工作是在亚太同步辐射研究机构神经科学联盟(SYNAPSE)的合作下进行的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a0b/9188605/571a077f2968/41598_2022_13377_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a0b/9188605/b422163383ad/41598_2022_13377_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a0b/9188605/536dbce713fb/41598_2022_13377_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a0b/9188605/f7bc3eb8e4b6/41598_2022_13377_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a0b/9188605/f9f6dcf0048e/41598_2022_13377_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a0b/9188605/bbf18c30ee43/41598_2022_13377_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a0b/9188605/571a077f2968/41598_2022_13377_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a0b/9188605/b422163383ad/41598_2022_13377_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a0b/9188605/536dbce713fb/41598_2022_13377_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a0b/9188605/f7bc3eb8e4b6/41598_2022_13377_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a0b/9188605/f9f6dcf0048e/41598_2022_13377_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a0b/9188605/bbf18c30ee43/41598_2022_13377_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a0b/9188605/571a077f2968/41598_2022_13377_Fig6_HTML.jpg

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