Iranian Center for Endodontic Research, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Curr Stem Cell Res Ther. 2024;19(4):587-610. doi: 10.2174/1574888X17666220609093939.
The current systematic review aims to provide the available evidence evaluating the biological interactions of dental stem cells (DSCs) and growth factor delivery systems.
Following the Preferred Reporting Items for a Systematic Reviews and Meta-Analyses (PRISMA) guidelines, systematic search was conducted in the electronic databases (PubMed/Medline, Scopus, Web of Science, and Google Scholar) up to January 2022. Studies evaluating the biological interactions of DSCs and growth factor delivery systems were included. The outcome measures were cell cytocompatibility, mineralization, and differentiation.
Sixteen studies were selected for the qualitative synthesis. The following growth factor delivery systems exhibit adequate cytocompatibility, enhanced mineralization, and osteo/odontoblast differentiation potential of DSCs: 1) Fibroblast growth factor (FGF-2)-loaded-microsphere and silk fibroin, 2) Bone morphogenic protein-2 (BMP-2)-loaded-microsphere and mesoporous calcium silicate scaffold, 3) Transforming growth factor Beta 1 (TGF-ß1)-loaded-microsphere, glass ionomer cement (GIC), Bio-GIC and liposome, 4) TGF-ß1-loaded-nanoparticles/scaffold, 5) Vascular endothelial growth factor (VEGF)-loaded-fiber and hydrogel, 6) TGF-ß1/VEGF-loaded-nanocrystalline calcium sulfate/hydroxyapatite/calcium sulfate, 7) Epidermal growth factor-loaded- nanosphere, 8) Stem cell factor/DSCs-loaded-hydrogel and Silk fibroin, 9) VEGF/BMP-2/DSCs-loaded-Three-dimensional matrix, 10) VEGF/DSCs-loaded-microsphere/hydrogel, and 11) BMP-2/DSCs and VEGF/DSCs-loaded-Collagen matrices. The included delivery systems showed viability, except for Bio-GIC on day 3. The choice of specific growth factors and delivery systems (, BMP-2-loaded-microsphere and VEGF-loaded-hydrogel) resulted in a greater gene expression.
This study, with low-level evidence obtained from studies, suggests that growth factor delivery systems induce cell proliferation, mineralization, and differentiation toward a therapeutic potential in regenerative endodontics.
本系统评价旨在提供现有评估牙源性干细胞（DSC）与生长因子递送系统生物学相互作用的证据。
根据系统评价和荟萃分析的首选报告项目（PRISMA）指南，对电子数据库（PubMed/Medline、Scopus、Web of Science 和 Google Scholar）进行了系统检索，检索时间截至 2022 年 1 月。纳入评估 DSC 与生长因子递送系统生物学相互作用的研究。结局指标为细胞细胞相容性、矿化和分化。
定性综合纳入了 16 项研究。以下生长因子递送系统显示出足够的细胞相容性、增强的矿化和 DSC 的成骨/成牙本质分化潜力：1）负载成纤维细胞生长因子（FGF-2）的微球和丝素蛋白，2）负载骨形态发生蛋白-2（BMP-2）的微球和介孔硅酸钙支架，3）转化生长因子β 1（TGF-β1）-负载微球、玻璃离子水泥（GIC）、生物 GIC 和脂质体，4）负载 TGF-β1 的纳米颗粒/支架，5）负载血管内皮生长因子（VEGF）的纤维和水凝胶，6）负载 TGF-β1/VEGF 的纳米晶硫酸钙/羟基磷灰石/硫酸钙，7）负载表皮生长因子的纳米球，8）干细胞因子/DSC 负载水凝胶和丝素蛋白，9）负载 VEGF/BMP-2/DSC 的三维基质，10）负载 VEGF/DSC 的微球/水凝胶，以及 11）负载 BMP-2/DSC 和 VEGF/DSC 的胶原基质。除第 3 天外，所有包含的递送系统均显示出细胞活力。选择特定的生长因子和递送系统（BMP-2 负载的微球和 VEGF 负载的水凝胶）导致基因表达增加。
本研究基于低水平证据的研究，表明生长因子递送系统诱导细胞增殖、矿化和向再生牙髓治疗潜力的分化。
