Iranian Center for Endodontic Research, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Curr Stem Cell Res Ther. 2024;19(4):523-543. doi: 10.2174/1574888X17666220628125048.
To evaluate the biological interaction between dental stem cells (DSCs) and different growth factors in the field of regenerative endodontics.
A systematic search was conducted in the electronic databases up to October 2021. This study followed the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. studies evaluating the biological interactions of DSCs and growth factors were included. The meta-analysis was performed according to the type of growth factor. The outcomes were cell viability/ proliferation and mineralization. Standardized mean differences (SMDs) were estimated using the random-effect maximum-likelihood method ( < .05). Additional analysis was performed to find any potential source of heterogeneity.
Twenty articles were included in the systematic review; meta-analysis was performed for fibroblast growth factor-2 (FGF-2) and Transforming growth factor-ß1 (TGF-β1) (n = 5). Results showed that use of FGF-2 significantly increased cell proliferation on day 1-(SMD = 3.56, = 0.00), 3-(SMD = 9.04, = 0.00), 5-(SMD = 8.37, = 0.01), and 7 (SMD=8.51, =0.00) than the control group. TGF-ß1 increased alkaline phosphatase (ALP) activity more than control only on day 3 (SMD = 3.68, = 0.02). TGF-β1 had no significant effect on cell proliferation on days 1 and 3 ( > 0.05) and on ALP activity on days 5 and 7 ( > 0.05). Meta-regression analysis showed that different covariates (., cell type, passage number, and growth factors' concentration) could significantly influence the effect sizes at different follow- ups ( < 0.05).
Specific growth factors might enhance the proliferation and mineralization of DSCs; however, the obtained evidence was weak. Due to the high heterogeneity among the included studies, other growth factors' inhibitory/stimulatory effects on DSCs could not be evaluated.
评估牙源性干细胞（DSC）与再生牙内科学领域不同生长因子之间的生物学相互作用。
系统检索截至 2021 年 10 月的电子数据库。本研究遵循系统评价和荟萃分析的首选报告项目（PRISMA）指南。纳入评估 DSC 和生长因子生物学相互作用的研究。根据生长因子的类型进行荟萃分析。使用随机效应最大似然法（ <.05）估计标准化均数差（SMD）。进行了额外分析以寻找任何潜在的异质性来源。
系统综述纳入 20 篇文章；对成纤维细胞生长因子-2（FGF-2）和转化生长因子-β1（TGF-β1）进行了荟萃分析（n = 5）。结果表明，与对照组相比，使用 FGF-2 可显著增加第 1 天（SMD = 3.56， = 0.00）、第 3 天（SMD = 9.04， = 0.00）、第 5 天（SMD = 8.37， = 0.01）和第 7 天（SMD=8.51， =0.00）的细胞增殖。TGF-β1 仅在第 3 天增加碱性磷酸酶（ALP）活性高于对照组（SMD = 3.68， = 0.02）。TGF-β1 对第 1 天和第 3 天的细胞增殖和第 5 天和第 7 天的 ALP 活性无显著影响（ > 0.05）。元回归分析表明，不同协变量（例如细胞类型、传代数和生长因子浓度）可能会在不同的随访时间内显著影响效应大小（ < 0.05）。
特定生长因子可能增强 DSC 的增殖和矿化，但获得的证据较弱。由于纳入研究的异质性较高，其他生长因子对 DSC 的抑制/刺激作用无法评估。
