Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University Hospital, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan.
Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan; 3D Printing Medical Research Institute, Asia University, Taichung, Taiwan.
J Endod. 2018 Nov;44(11):1677-1685. doi: 10.1016/j.joen.2018.08.008.
Calcium silicate bioceramics have been broadly used as reparative or grafting materials with good bioactivity and biocompatibility in dental application. It has been shown that applying a mesoporous process to calcium silicate gives it great potential as a controlled drug delivery system.
The aim of this study was to investigate a novel osteoinductive scaffold by loading bone morphogenetic protein 2 (BMP-2) to mesoporous calcium silicate (MesoCS) and fabricating it as 3-dimensional scaffolds using fused deposition modeling combined with polycaprolactone.
The MesoCS/BMP-2 scaffold showed similar patterns to that of a calcium silicate scaffold in releasing calcium and silicon ions in a simulated body fluid (SBF) immersion test for 7 days, but BMP-2 continued releasing from the MesoCS/BMP-2 scaffold significantly more than the CS scaffold from 48 hours to 7 days. Adhesion and proliferation of human dental pulp cells cultured on a MesoCS/BMP-2 scaffold were also more significant than scaffolds without BMP-2 or mesoporous as well as the results of the test on alkaline phosphatase activity.
The results support that the novel 3-dimensional-printed MesoCS scaffold performed well as BMP-2 delivery system and would be an ideal odontoinductive biomaterial in regenerative endodontics.
硅酸钙生物陶瓷在牙科应用中具有良好的生物活性和生物相容性，已广泛用作修复或移植材料。研究表明，对硅酸钙进行介孔处理使其具有作为控释药物输送系统的巨大潜力。
本研究旨在通过将骨形态发生蛋白 2（BMP-2）加载到介孔硅酸钙（MesoCS）中，并用熔融沉积建模结合聚己内酯制备成 3D 支架，从而构建一种新型的诱导成骨支架。
在模拟体液（SBF）浸泡测试的第 7 天，MesoCS/BMP-2 支架在释放钙和硅离子方面表现出与硅酸钙支架相似的模式，但从第 48 小时到第 7 天，BMP-2 从 MesoCS/BMP-2 支架中的释放量明显高于 CS 支架。人牙髓细胞在 MesoCS/BMP-2 支架上的黏附和增殖也比没有 BMP-2 或介孔的支架以及碱性磷酸酶活性测试的结果更显著。
结果表明，新型 3D 打印 MesoCS 支架作为 BMP-2 输送系统表现良好，将成为再生牙髓学中理想的诱导成骨生物材料。
