Department of Neuromedicine and Movement Science, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), 7030 Trondheim, Norway.
Department of Clinical Neuroscience, Division of Neuro, Head, and Neck, Umeå University Hospital, 907 37 Umeå, Sweden.
STAR Protoc. 2022 Jun 7;3(2):101426. doi: 10.1016/j.xpro.2022.101426. eCollection 2022 Jun 17.
Here, we present a unified protocol for the extraction, culture, and basic characterization of rat neural stem cells (NSCs) from all three canonical neurogenic niches in the brain and spinal cord. We describe tissue dissection and dissociation, cell culture, followed by EdU labeling and characterization of NSCs. By yielding considerable numbers of viable cells per animal, this protocol enables the establishment of substantial, long-term cell banks, thus offering cost and labor efficiency while significantly reducing the numbers of animals used.
在这里，我们提出了一个从大脑和脊髓的所有三个典型神经发生龛中提取、培养和基本鉴定大鼠神经干细胞（NSC）的统一方案。我们描述了组织解剖和分离、细胞培养，然后进行 EdU 标记和 NSC 鉴定。该方案从每只动物中获得大量的活细胞，从而能够建立大量的长期细胞库，因此提高了成本和劳动效率，同时显著减少了动物的使用数量。
