Deparment of Urology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Baxter Laboratory for Stem Cell Biology, Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
STAR Protoc. 2022 Jun 7;3(2):101425. doi: 10.1016/j.xpro.2022.101425. eCollection 2022 Jun 17.
Trogocytosis is an active transport mechanism by which one cell extracts a plasma membrane fragment with embedded molecules from an adjacent cell in a contact-dependent process leading to the acquisition of a new function. Our protocol, which has general applicability, consolidates and optimizes existing protocols while highlighting key experimental variables to demonstrate that natural killer (NK) cells acquire the tetraspanin CD9 by trogocytosis from ovarian tumor cells. For complete details on the use and execution of this protocol, please refer to Gonzalez et al. (2021).
细胞融合是一种主动运输机制，通过该机制，一个细胞在接触依赖性过程中从相邻细胞中提取带有嵌入式分子的质膜片段，从而获得新的功能。我们的方案具有普遍适用性，它整合和优化了现有的方案，同时突出了关键的实验变量，以证明自然杀伤 (NK) 细胞通过从卵巢肿瘤细胞中发生细胞融合获得四跨膜蛋白 CD9。有关该方案使用和执行的完整详细信息，请参见 Gonzalez 等人。（2021 年）。
