Department of Neurology, Center for Advanced Research on Diagnostic Assays, Icahn School of Medicine at Mount Sinai (ISMMS), New York, NY, USA.
STAR Protoc. 2022 Jun 7;3(2):101446. doi: 10.1016/j.xpro.2022.101446. eCollection 2022 Jun 17.
Concomitant profiling of transcriptome and chromatin accessibility in isolated nuclei can reveal gene regulatory control mechanisms in health and disease. We report a single nucleus multi-omics analysis protocol optimized for frozen archived postmortem human pituitaries that is also effective for frozen ovine and murine pituitaries and human skeletal muscle biopsies. Its main advantages are that (1) it is not limited to fresh tissue, (2) it avoids tissue dissociation-induced transcriptional changes, and (3) it includes a novel, automated quality control pipeline. For complete details on the use and execution of this protocol, please refer to Ruf-Zamojski et al. (2021) and Zhang et al. (2022).
在分离的细胞核中同时进行转录组和染色质可及性分析，可以揭示健康和疾病状态下的基因调控控制机制。我们报告了一种优化的用于冷冻存档的人垂体的单个细胞核多组学分析方案，该方案对于冷冻的绵羊和鼠垂体以及人骨骼肌活检也同样有效。其主要优点是：(1) 不受限于新鲜组织；(2) 避免了组织解离诱导的转录变化；(3) 包括一个新颖的、自动化的质量控制流程。有关该方案的使用和执行的完整详细信息，请参考 Ruf-Zamojski 等人（2021 年）和 Zhang 等人（2022 年）。
