黄精赞育胶囊治疗少弱精子症的代谢组学分析及氧化应激缓解作用
Metabolomic profiling and alleviation of oxidative stress of Huangjing Zanyu capsule treating oligoasthenospermia.
作者信息
Li Boyi, Ma Jinchen, Zhu Linghui, Yan Yun, Wu Yuqi, Zheng Chenglong, Wu Zhisheng, Li Lingru, Wang Qi, Zheng Yanfei
机构信息
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
出版信息
Transl Androl Urol. 2022 May;11(5):659-674. doi: 10.21037/tau-22-293.
BACKGROUND
The traditional Chinese medicine (TCM) patent medicine Huangjing Zanyu capsule (HJZY capsule) has achieved satisfactory clinical effects in the treatment of oligoasthenospermia (OAS). This study aimed to elucidate the impact of HJZY capsule on the reproductive system, focusing on oxidative stress and metabolism profiling during the intervention, to clarify the therapeutic mechanism of HJZY capsule in treating OAS.
METHODS
Cyclophosphamide was used to establish OAS model rats. Time-sequence specimen collection was applied to monitor the dynamic development of the pharmacological effect of HJZY capsule. Superoxide dismutase (SOD), glutathione peroxidase (GPX), and malonaldehyde (MDA) were evaluated by biochemistry kits to examine the impact of HJZY capsule on oxidative stress. Non-targeted metabolomics was conducted for urine and testis samples, respectively, to investigate metabolic pathways through which the HJZY capsule takes effect.
RESULTS
The HJZY capsule elevated sperm density from 62.1±8.28, passing 68.4±7.52, to 75.9±8.48×10/mL, and sperm motility from 62.0%±3.94%, passing 70.8%±9.72%, to 68.8%±4.37%. Meanwhile, SOD (P<0.05 in week 2) and GPX activity levels of HJZY groups were elevated to a certain degree, respectively, and lipid oxidation was attenuated, as shown by decreased MDA content (P<0.05 in week 2). Metabolomics results showed that the HJZY capsule could modulate pathways including taurine metabolism, purine and pyrimidine metabolism, glycerolipid and glycerophospholipid metabolism, and multiple amino acid metabolisms, among others. The cluster analysis results showed that urinary and testicular metabolomics differed in the strength of discrimination between rats in the OAS model and the HJZY groups.
CONCLUSIONS
The HJZY capsule exerts a comprehensive effect on OAS through influencing various metabolic pathways. Non-targeted metabolomics provides an effective way for profiling complex TCM prescriptions.
背景
中药专利药黄精赞育胶囊（HJZY胶囊）在少弱精子症（OAS）治疗中取得了满意的临床效果。本研究旨在阐明HJZY胶囊对生殖系统的影响，重点关注干预过程中的氧化应激和代谢谱，以阐明HJZY胶囊治疗OAS的作用机制。
方法
采用环磷酰胺建立OAS模型大鼠。应用时间序列标本采集来监测HJZY胶囊药理作用的动态发展。通过生化试剂盒评估超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）和丙二醛（MDA），以检测HJZY胶囊对氧化应激的影响。分别对尿液和睾丸样本进行非靶向代谢组学研究，以探究HJZY胶囊发挥作用的代谢途径。
结果
HJZY胶囊使精子密度从62.1±8.28提升至75.9±8.48×10/mL，精子活力从62.0%±3.94%提升至68.8%±4.37%。同时，HJZY组的SOD（第2周P<0.05）和GPX活性水平分别有一定程度升高，脂质氧化减弱（第2周MDA含量降低，P<0.05）。代谢组学结果显示HJZY胶囊可调节包括牛磺酸代谢、嘌呤和嘧啶代谢、甘油脂质和甘油磷脂代谢以及多种氨基酸代谢等途径。聚类分析结果显示OAS模型大鼠和HJZY组大鼠尿液和睾丸代谢组学在区分强度上存在差异。
结论
HJZY胶囊通过影响多种代谢途径对OAS发挥综合作用效果。非靶向代谢组学为剖析复杂的中药方剂提供了有效方法。
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