麒麟丸对少弱精子症大鼠生精功能、生殖激素、氧化应激及 TSSK2 基因的影响
Effects of Qilin pills on spermatogenesis, reproductive hormones, oxidative stress, and the TSSK2 gene in a rat model of oligoasthenospermia.
机构信息
Department of Reproductive Medicine, the Affiliated Hospital of Qingdao University, Qingdao, 266000, China.
National Health and Family Planning Key Laboratory of Male Reproductive Health, Department of Male Clinical Research, National Research Institute for Family Planning & WHO Collaborating Center for Research in Human Reproduction, Beijing, 100081, China.
出版信息
BMC Complement Med Ther. 2020 Feb 11;20(1):42. doi: 10.1186/s12906-019-2799-7.
BACKGROUND
Qilin pills (QLPs), a classic Traditional Chinese Medicine (TCM) formula for treating male infertility, effectively improve semen quality in clinical trials. This study was designed to evaluate the effects of QLPs on spermatogenesis, reproductive hormones, oxidative stress, and the testis-specific serinekinase-2 (TSSK2) gene in a rat model of oligoasthenospermia.
METHODS
Forty adult male Sprague-Dawley (SD) rats were randomly divided into four groups. The rat model with oligoasthenospermia was generated by intragastric administration of tripterygium glycosides (TGs) once daily for 4 weeks. Then, two treatment groups were given different doses (1.62 g/kg and 3.24 g/kg) of QLPs once daily for 60 days. Sperm parameters, testicular histology and reproductive hormone measurements, oxidative stress tests, and TSSK2 expression tests were carried out.
RESULTS
QLPs effectively improved semen parameters and testicular histology; restored the levels of FSH, LH, PRL, fT, and SHBG; reduced the levels of oxidative stress products (ROS and MDA); increased testicular SOD activity; and restored the expression of spermatogenesis-related gene TSSK2.
CONCLUSION
QLPs have a therapeutic effect on a rat model of oligoasthenospermia, and this effect is manifested as improvement of semen quality and testis histology, gonadal axis stability, decreased oxidative stress, and the regulation of testis-specific spermatogenesis-related gene TSSK2.
背景
麒麟丸（QLP）是一种治疗男性不育的经典中药方剂，在临床试验中能有效改善精液质量。本研究旨在评估 QLP 对少弱精子症大鼠模型的生精作用、生殖激素、氧化应激和睾丸特异性丝氨酸激酶-2（TSSK2）基因的影响。
方法
40 只成年雄性 Sprague-Dawley（SD）大鼠随机分为 4 组。通过每日灌胃雷公藤多苷（TGs）建立少弱精子症大鼠模型，连续 4 周。然后，两组治疗组分别给予不同剂量（1.62 g/kg 和 3.24 g/kg）的 QLP 每日 1 次，连续 60 天。检测精子参数、睾丸组织学和生殖激素水平、氧化应激试验、TSSK2 表达试验。
结果
QLP 能有效改善精子参数和睾丸组织学；恢复 FSH、LH、PRL、fT 和 SHBG 水平；降低氧化应激产物（ROS 和 MDA）水平；增加睾丸 SOD 活性；恢复与精子发生相关的基因 TSSK2 的表达。
结论
QLP 对少弱精子症大鼠模型具有治疗作用，其作用表现为改善精液质量和睾丸组织学、性腺轴稳定性、降低氧化应激和调节睾丸特异性与精子发生相关的基因 TSSK2。
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