Tompa P, Batke J, Ovádi J
FEBS Lett. 1987 Apr 20;214(2):244-8. doi: 10.1016/0014-5793(87)80063-7.
A kinetic method, based upon measuring the transient time of coupled reactions, is proposed for the determination of the intermediate channel efficiency in a system of functionally interacting enzymes. The procedure rests upon a novel description in which the transient time is expressed as a function of channel efficiency and lifetime of the intermediate molecules. By this approach the reduction of transient time can be explained even if no changes in the kinetic parameters of the individual reactions occur. For determining channel efficiency, a linearized form has been evaluated and applied to the analysis of the kinetics of the aspartate aminotransferase-glutamate dehydrogenase coupled reaction, for which the data were taken from the literature [(1982) Eur. J. Biochem. 121, 511-517].
提出了一种基于测量偶联反应瞬态时间的动力学方法，用于测定功能相互作用酶系统中的中间通道效率。该方法基于一种新颖的描述，其中瞬态时间表示为通道效率和中间分子寿命的函数。通过这种方法，即使各个反应的动力学参数没有变化，也可以解释瞬态时间的减少。为了确定通道效率，已经评估了一种线性化形式，并将其应用于天冬氨酸转氨酶-谷氨酸脱氢酶偶联反应的动力学分析，其数据取自文献[(1982) Eur. J. Biochem. 121, 511-517]。
