Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Neustadt am Ruebenberge, Germany.
Methods Mol Biol. 2022;2495:275-293. doi: 10.1007/978-1-0716-2301-5_15.
In livestock industry, one sex is usually preferred over the other due to its impact on the production (e.g., milk from cows, eggs from laying hens, or meat from bulls). Boar taint, to which most of the consumers are susceptible, is a major challenge for the pork industry in the light of the enacted ban of castration without anesthesia from 2021 in Germany. Consequently, a shift towards an increased female ratio would be of great benefit for the pork production. We recently described that a CRISPR/Cas9-mediated knockout of the porcine SRY gene by intracytoplasmic microinjection or SCNT resulted in genetically male pigs with a female phenotype. This sex reversal study in pigs revealed a pivotal role of the SRY gene in male sex determination and might pave the way for the generation of boars that produce only female offspring.
在畜牧业中，由于其对生产的影响（例如，奶牛的牛奶、蛋鸡的鸡蛋或公牛的肉），通常会偏爱一种性别。由于 2021 年在德国颁布了禁止未经麻醉去势的法案，大多数消费者都容易受到猪腥味的影响，这对猪肉行业来说是一个重大挑战。因此，增加雌性比例将对猪肉生产有很大的好处。我们最近描述了通过细胞质内显微注射或体细胞核移植的 CRISPR/Cas9 介导的猪 SRY 基因敲除导致具有雌性表型的遗传雄性猪。这项在猪中的性别逆转研究揭示了 SRY 基因在雄性性别决定中的关键作用，并可能为产生只产生雌性后代的公猪铺平道路。
