Li Pengtao, Liu Qiankun, Wei Yangyang, Xing Chaozhu, Xu Zhongping, Ding Fang, Liu Yuling, Lu Quanwei, Hu Nan, Wang Tao, Zhu Xiangqian, Cheng Shuang, Li Zhaoguo, Zhao Zilin, Li Yanfang, Han Jiangping, Cai Xiaoyan, Zhou Zhongli, Wang Kunbo, Zhang Baohong, Liu Fang, Jin Shuangxia, Peng Renhai
College of Biology and Food Engineering, Anyang Institute of Technology, Anyang, Henan, 455000, China; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, 455000, China; Research Base, Anyang Institute of Technology, State Key Laboratory of Cotton Biology, Anyang 455000, Henan, China.
College of Biology and Food Engineering, Anyang Institute of Technology, Anyang, Henan, 455000, China; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, 455000, China; School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, China.
Plant Commun. 2023 Oct 27;5(2):100740. doi: 10.1016/j.xplc.2023.100740.
Increasing soil salinization has led to severe losses of plant yield and quality. Thus, it is urgent to investigate the molecular mechanism of the salt stress response. In this study, we took systematically analyzed cotton root response to salt stress by single-cell transcriptomics technology; 56,281 high-quality cells were totally obtained from 5-days-old lateral root tips of Gossypium arboreum under natural growth and different salt-treatment conditions. Ten cell types with an array of novel marker genes were synthetically identified and confirmed with in situ RNA hybridization, and some specific-type cells of pesudotime analysis also pointed out their potential differentiation trajectory. The prominent changes of cell numbers responding to salt stress were observed on outer epidermal and inner endodermic cells, which were significantly enriched in response to stress, amide biosynthetic process, glutathione metabolism, and glycolysis/gluconeogenesis. Other functional aggregations were concentrated on plant-type primary cell wall biogenesis, defense response, phenylpropanoid biosynthesis and metabolic pathways by analyzing the abundant differentially expressed genes (DEGs) identified from multiple comparisons. Some candidate DEGs related with transcription factors and plant hormones responding to salt stress were also identified, of which the function of Ga03G2153, an annotated auxin-responsive GH3.6, was confirmed by using virus-induced gene silencing (VIGS). The GaGH3.6-silenced plants presented severe stress-susceptive phenotype, and suffered more serious oxidative damages by detecting some physiological and biochemical indexes, indicating that GaGH3.6 might participate in salt tolerance in cotton through regulating oxidation-reduction process. For the first time, a transcriptional atlas of cotton roots under salt stress were characterized at a single-cell resolution, which explored the cellular heterogeneityand differentiation trajectory, providing valuable insights into the molecular mechanism underlying stress tolerance in plants.
土壤盐渍化加剧导致植物产量和品质严重下降。因此，迫切需要研究盐胁迫响应的分子机制。在本研究中，我们通过单细胞转录组学技术系统分析了棉花根系对盐胁迫的响应；从自然生长和不同盐处理条件下的5日龄陆地棉侧根根尖共获得56,281个高质量细胞。综合鉴定出十种具有一系列新标记基因的细胞类型，并通过原位RNA杂交进行了验证，一些拟时间分析的特定类型细胞也指出了它们潜在的分化轨迹。在外侧表皮细胞和内侧内胚层细胞中观察到响应盐胁迫的细胞数量有显著变化，这些细胞在胁迫响应、酰胺生物合成过程、谷胱甘肽代谢和糖酵解/糖异生方面显著富集。通过分析从多个比较中鉴定出的大量差异表达基因（DEG），其他功能聚集集中在植物型初生细胞壁生物合成、防御反应、苯丙烷生物合成和代谢途径上。还鉴定了一些与响应盐胁迫的转录因子和植物激素相关的候选DEG，其中通过病毒诱导基因沉默（VIGS）证实了一个注释为生长素响应型GH3.6的Ga03G2153的功能。沉默GaGH3.6的植株表现出严重的胁迫敏感表型，通过检测一些生理生化指标发现其遭受了更严重的氧化损伤，表明GaGH3.6可能通过调节氧化还原过程参与棉花的耐盐性。首次以单细胞分辨率描绘了盐胁迫下棉花根系的转录图谱，探索了细胞异质性和分化轨迹，为植物胁迫耐受性的分子机制提供了有价值的见解。
