School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, Sichuan, China.
College of Pharmacy, Shenzhen Technology University, Shenzhen 518118, Guangdong, China.
J Pharm Biomed Anal. 2022 Sep 5;218:114861. doi: 10.1016/j.jpba.2022.114861. Epub 2022 Jun 1.
Chrysosplenium is the main component of a variety of Tibetan prescription preparations. Nevertheless, there are few chemical reports for different species of Chrysosplenium, which should be further explored. To this end, ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UPLC-Q Exactive Orbitrap HRMS) and high-performance liquid chromatography-diode array detection (HPLC-DAD) were first integrated to qualitatively analyse the chemical characteristics of Chrysosplenium nudicaule, Chrysosplenium carnosum, Chrysosplenium sikangense, Chrysosplenium griffithii, Chrysosplenium absconditicapsulum, Chrysosplenium forrestii and Chrysosplenium axillare. As a result, a total of 40 compounds were identified or tentatively identified from these 7 species of Chrysosplenium, including 21 flavonoids, 3 triterpenoids and a variety of alkaloids, organic acids and anthraquinones, etc. Among them, 6 compounds were detected for the first time, and 8 compounds are common components in all 7 species of Chrysosplenium. In the specific chromatogram, 4 characteristic peaks, namely Riboflavin, 5,4'-dihydroxy-3,6,3'-trimethoxyflavin-7-O-β-D-glucoside, 5,7,3'-trihydroxy-6,4',5'-trimethoxyflavone and Chrysosplenetin, were selected to evaluate the similarities of 17 batches of Chrysosplenium samples, which ranged from 0.770 to 0.994. The established method is simple, feasible and accurate, and was proven to be suitable for characterizing the chemical compositions of Chrysosplenium from different species and evaluating their similarities by specific chromatogram analysis to clarify the rationality of using Chrysosplenium from different species in clinical medication, which provides experimental data for further quality evaluation of Chrysosplenium.
獐牙菜属是多种藏药制剂的主要成分。然而，对于不同獐牙菜属物种的化学成分研究较少，有待进一步探索。为此，首次采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱（UPLC-Q Exactive Orbitrap HRMS）和高效液相色谱-二极管阵列检测（HPLC-DAD）联用技术对獐牙菜属 7 种植物（獐牙菜、川西獐牙菜、四棱獐牙菜、川西獐牙菜、獐牙菜、毛獐牙菜和藏獐牙菜）的化学成分进行了定性分析。结果共鉴定或推测出 7 种獐牙菜属植物中的 40 种化合物，包括 21 种黄酮类化合物、3 种三萜类化合物和多种生物碱、有机酸和蒽醌类化合物等。其中，首次检测到 6 种化合物，8 种化合物为 7 种獐牙菜属植物的共有成分。在特定的色谱图中，选择了 4 个特征峰，即核黄素、5,4'-二羟基-3,6,3'-三甲氧基黄酮-7-O-β-D-葡萄糖苷、5,7,3'-三羟基-6,4',5'-三甲氧基黄酮和獐牙菜苦苷，用于评价 17 批獐牙菜属样品的相似度，范围为 0.770-0.994。该方法简单、可行、准确，通过特征色谱图分析证明适用于不同獐牙菜属植物化学成分的表征和相似度评价，以明确临床用药中使用不同獐牙菜属植物的合理性，为獐牙菜属的进一步质量评价提供实验数据。
