College of Traditional Chinese Medicine Resources, Guangdong Pharmaceutical University, Yunfu 527325, China.
Experimental Center of Yunfu Campus, Guangdong Pharmaceutical University, Yunfu 527325, China.
Molecules. 2023 Jun 1;28(11):4504. doi: 10.3390/molecules28114504.
(L.) DC., a perennial herb in the Asteraceae family native to China and Southeast Asia, has a notable history of medicinal use due to its pharmacological properties. Using UPLC-Q-Orbitrap HRMS techniques, we systematically investigated the chemical constituents of this plant. A total of 31 constituents were identified, of which 14 were flavonoid compounds. Significantly, 18 of these compounds were identified in for the first time. Furthermore, the mass spectrometry fragmentation patterns of significant chemical constituents identified in were analyzed, providing important insights into their structural characteristics. The in vitro antioxidative potential of the methanol extract of was assessed using DPPH and ABTS free-radical-scavenging assays, total antioxidative capacity, and reducing power. The antioxidative activity exhibited a direct correlation with the mass concentration of the extract, with IC values of 105.1 ± 0.503 μg/mL and 12.49 ± 0.341 μg/mL for DPPH and ABTS, respectively. For total antioxidant capacity, the absorbance was 0.454 ± 0.009 at 400 μg/mL. In addition, the reducing power was 1.099 ± 0.03 at 2000 μg/mL. This study affirms that UPLC-Q-Orbitrap HRMS can effectively discern the chemical constituents in , primarily its flavonoid compounds, and substantiates its antioxidative properties. This underscores its potential utility as a natural antioxidant in the food, pharmaceutical, and cosmetics sectors. This research provides a valuable theoretical basis and reference value for the comprehensive development and utilization of and expands our understanding of this medicinally valuable plant.
（L.）DC.，一种原产于中国和东南亚的菊科多年生草本植物，由于其药理学特性，具有显著的药用历史。我们使用 UPLC-Q-Orbitrap HRMS 技术系统地研究了这种植物的化学成分。共鉴定出 31 种成分，其中 14 种为黄酮类化合物。值得注意的是，其中 18 种化合物是首次在 中鉴定出来的。此外，还分析了 中鉴定出的重要化学成分的质谱碎裂模式，为其结构特征提供了重要的见解。采用 DPPH 和 ABTS 自由基清除实验、总抗氧化能力和还原力评估了 甲醇提取物的体外抗氧化潜力。抗氧化活性与提取物的质量浓度呈直接相关，DPPH 和 ABTS 的 IC 值分别为 105.1 ± 0.503 μg/mL 和 12.49 ± 0.341 μg/mL。对于总抗氧化能力，在 400 μg/mL 时吸光度为 0.454 ± 0.009。此外，还原力在 2000 μg/mL 时为 1.099 ± 0.03。本研究证实，UPLC-Q-Orbitrap HRMS 可以有效地辨别 中的化学成分，主要是其黄酮类化合物，并证实了其抗氧化特性。这突出了它作为食品、制药和化妆品行业天然抗氧化剂的潜在用途。这项研究为 的综合开发利用提供了有价值的理论基础和参考价值，并扩展了我们对这种具有药用价值的植物的认识。
