Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.
Bioresour Technol. 2022 Sep;359:127460. doi: 10.1016/j.biortech.2022.127460. Epub 2022 Jun 11.
The diatom Phaeodactylum tricornutum is considered a promising "cell factory" for high-value products fucoxanthin and EPA. But its potential has not been realized due largely to microbial contamination. In this study, seven protozoan strains were identified, of which a heterolobosean amoeba was identified as the most frequently occurring and destructive predator in P. tricornutum culture. The addition of 400 mg L NHHCO inhibited amoeba proliferation with little impact on algal growth. Halting culture mixing at night induced a hypoxia environment that further inhibited amoeba growth. Regardless of culture systems employed, a periodical supply of proper amounts of NHHCO alone or in combination with halting culture mixing at night may prevent or treat protozoa contamination in mass culture of P. tricornutum.
三角褐指藻被认为是一种很有前途的“细胞工厂”，可以生产高价值的产品——岩藻黄质和 EPA。但是，由于微生物污染，它的潜力尚未得到实现。在这项研究中，鉴定出了 7 种原生动物株，其中一种异变形阿米巴虫被鉴定为三角褐指藻培养物中最常出现和最具破坏性的捕食者。添加 400mg/L 的 NHHCO 可以抑制阿米巴虫的增殖，而对藻类的生长影响很小。夜间停止培养混合会诱导缺氧环境，进一步抑制阿米巴虫的生长。无论采用哪种培养系统，定期单独或结合夜间停止培养混合供应适量的 NHHCO 都可能防止或治疗三角褐指藻大规模培养中的原生动物污染。
