College of Resources and Environment, Shandong Agricultural University, Key Laboratory of Agricultural Environment in Universities of Shandong, 61 Daizong Road, Taian 271018, PR China.
College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2022 Sep;259:109394. doi: 10.1016/j.cbpc.2022.109394. Epub 2022 Jun 11.
The present study utilized a biomarker response method to evaluate the effect of 3,5,6-trichloro-2-pyridinol (TCP) in artificial and natural soils on Eisenia fetida after 7, 14, 28, 42 and 56 days exposure. Results indicated that TCP induced excessive reactive oxygen species, caused oxidative stress and DNA damage to Eisenia fetida. Biomarker responses were standardized to calculate the Integrated Biomarker Response (IBR) index. The IBR index of three enzymes (superoxide dismutase, catalase and glutathione S-transferase) activities showed that TCP induced the oxidative stress to E. fetida in red clay was stronger than in the other three soils. Specifically, chlorpyrifos exposure group showed a lower toxicity than TCP exposure group after 28 days exposure but a higher toxicity than TCP exposure group after 56 days exposure. Despite the deficiencies of this study, the above information is of great significance for assessing the risk of chlorpyrifos and its metabolite TCP pollution in soil ecosystems.
本研究采用生物标志物响应方法，评估了 3,5,6-三氯-2-吡啶醇（TCP）在人工和自然土壤中对赤子爱胜蚓暴露 7、14、28、42 和 56 天后的影响。结果表明，TCP 诱导赤子爱胜蚓产生过量的活性氧，导致氧化应激和 DNA 损伤。将生物标志物反应标准化以计算综合生物标志物反应（IBR）指数。三种酶（超氧化物歧化酶、过氧化氢酶和谷胱甘肽 S-转移酶）活性的 IBR 指数表明，TCP 对红黏土中赤子爱胜蚓的氧化应激作用强于其他三种土壤。具体来说，在暴露 28 天后，氯吡硫磷暴露组的毒性低于 TCP 暴露组，但在暴露 56 天后，氯吡硫磷暴露组的毒性高于 TCP 暴露组。尽管本研究存在局限性，但上述信息对于评估土壤生态系统中氯吡硫磷及其代谢物 TCP 污染的风险具有重要意义。
