College of Resources and Environment, Shandong Agricultural University, Key Laboratory of Agricultural Environment in Universities of Shandong, National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Taian 271018, China.
College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2023 Jan;263:109485. doi: 10.1016/j.cbpc.2022.109485. Epub 2022 Oct 8.
Atrazine has been widely used in the world and caused environmental pollution, especially soil pollution. When assessing the toxicity of atrazine in soil, most studies used standardized artificial soils, while few studies focused on the real soil environments. In the present study, three natural soils and artificial soil were selected as test soils to study and compare the toxicities of atrazine to Eisenia fetida. Acute toxicity of atrazine was determined by filter paper and soil tests. In chronic toxicity study, after atrazine exposure, the content of reactive oxygen species in Eisenia fetida significantly increased and showed a dose-response relationship. The activity changes of three antioxidant enzymes and glutathione transferase showed that atrazine had obvious oxidative stress effect on earthworms. The contents of malondialdehyde and 8-hydroxy deoxyguanosine in 0.1 and 1 mg/kg atrazine treatment groups were significantly higher than the control, indicating that medium and high concentrations of atrazine could cause lipid and DNA damage in Eisenia fetida. The acute toxicity results and the integrated biomarker response index for chronic toxicity indicated that the toxicity order of atrazine was: red clay > fluvo-aquic soil > artificial soil > black soil, and that the toxicity of atrazine in artificial soil was not representative of its toxicity in real soil environment. The results of correlation analysis showed that three soil property parameters of organic carbon, organic matter and sand were most related to the toxicity of atrazine.
莠去津在世界范围内被广泛使用，造成了环境污染，尤其是土壤污染。在评估莠去津在土壤中的毒性时，大多数研究使用标准化的人工土壤，而很少有研究关注真实的土壤环境。在本研究中，选择了三种天然土壤和人工土壤作为测试土壤，以研究和比较莠去津对赤子爱胜蚓的毒性。通过滤纸和土壤测试法测定莠去津的急性毒性。在慢性毒性研究中，暴露于莠去津后，赤子爱胜蚓体内活性氧物质的含量显著增加，表现出剂量-反应关系。三种抗氧化酶和谷胱甘肽转移酶的活性变化表明莠去津对蚯蚓具有明显的氧化应激作用。在 0.1 和 1 mg/kg 莠去津处理组中，丙二醛和 8-羟基脱氧鸟苷的含量明显高于对照组，表明中高浓度莠去津可导致赤子爱胜蚓的脂质和 DNA 损伤。急性毒性结果和慢性毒性的综合生物标志物反应指数表明莠去津的毒性顺序为：红黏土>潮土>人工土壤>黑土，人工土壤中莠去津的毒性不能代表其在真实土壤环境中的毒性。相关分析结果表明，有机碳、有机质和砂粒三种土壤性质参数与莠去津的毒性最相关。
