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薰衣草转录因子 LaMYC4 调控挥发性萜类生物合成。

The transcription factor LaMYC4 from lavender regulates volatile Terpenoid biosynthesis.

机构信息

Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Xiangshan, Beijing, 100093, China.

University of Chinese Academy of Sciences, Beijing, 100015, China.

出版信息

BMC Plant Biol. 2022 Jun 13;22(1):289. doi: 10.1186/s12870-022-03660-3.

DOI:10.1186/s12870-022-03660-3
PMID:35698036
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9190104/
Abstract

BACKGROUND

The basic helix-loop-helix (bHLH) transcription factors (TFs), as one of the largest families of TFs, are essential regulators of plant terpenoid biosynthesis and response to stresses. Lavender has more than 75 volatile terpenoids, yet few TFs have been identified to be involved in the terpenoid biosynthesis.

RESULTS

Based on RNA-Seq, reverse transcription-quantitative polymerase chain reaction, and transgenic technology, this study characterized the stress-responsive transcription factor LaMYC4 regulates terpenoid biosynthesis. Methyl jasmonate (MeJA) treatment increased volatile terpenoid emission, and the differentially expressed gene LaMYC4 was isolated. LaMYC4 expression level was higher in leaf than in other tissues. The expression of LaMYC4 decreased during flower development. The promoter of LaMYC4 contained hormone and stress-responsive regulatory elements and was responsive to various treatments, including UV, MeJA treatment, drought, low temperature, Pseudomonas syringae infection, and NaCl treatment. LaMYC4 overexpression increased the levels of sesquiterpenoids, including caryophyllenes, in Arabidopsis and tobacco plants. Furthermore, the expression of crucial node genes involved in terpenoid biosynthesis and glandular trichome number and size increased in transgenic tobacco.

CONCLUSIONS

We have shown that the stress-responsive MYC TF LaMYC4 from 'Jingxun 2' lavender regulates volatile terpenoid synthesis. This study is the first to describe the cloning of LaMYC4, and the results help understand the role of LaMYC4 in terpenoid biosynthesis.

摘要

背景

碱性螺旋-环-螺旋转录因子（bHLH）作为最大的转录因子家族之一，是植物萜类生物合成和应激反应的重要调节因子。薰衣草含有超过 75 种挥发性萜类化合物，但参与萜类生物合成的转录因子很少。

结果

基于 RNA-Seq、反转录定量聚合酶链式反应和转基因技术，本研究鉴定了应激响应转录因子 LaMYC4 调控萜类生物合成。茉莉酸甲酯（MeJA）处理增加了挥发性萜类化合物的排放，并分离出差异表达基因 LaMYC4。LaMYC4 的表达水平在叶片中高于其他组织。在花发育过程中，LaMYC4 的表达水平降低。LaMYC4 启动子含有激素和应激响应调控元件，对各种处理（包括 UV、MeJA 处理、干旱、低温、丁香假单胞菌感染和 NaCl 处理）均有响应。LaMYC4 的过表达增加了拟南芥和烟草植物中倍半萜烯的含量，包括石竹烯。此外，与萜类生物合成相关的关键节点基因的表达和腺毛的数量和大小在转基因烟草中增加。

结论

我们已经表明，来自‘静勋 2 号’薰衣草的应激响应 MYC TF LaMYC4 调节挥发性萜类化合物的合成。本研究首次描述了 LaMYC4 的克隆，结果有助于了解 LaMYC4 在萜类生物合成中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/255204e418fc/12870_2022_3660_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/4e907ee52e6f/12870_2022_3660_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/288b66d97d2d/12870_2022_3660_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/a425bb3d9c31/12870_2022_3660_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/2f6dc4e7ee3b/12870_2022_3660_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/5ce02561fde7/12870_2022_3660_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/704ea3029b8d/12870_2022_3660_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/e25f645ffc05/12870_2022_3660_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/255204e418fc/12870_2022_3660_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/4e907ee52e6f/12870_2022_3660_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/288b66d97d2d/12870_2022_3660_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/a425bb3d9c31/12870_2022_3660_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/2f6dc4e7ee3b/12870_2022_3660_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/5ce02561fde7/12870_2022_3660_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/704ea3029b8d/12870_2022_3660_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/e25f645ffc05/12870_2022_3660_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2c1/9190104/255204e418fc/12870_2022_3660_Fig8_HTML.jpg

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