模拟饲养条件下的全长转录组测序揭示了参与防御反应的bHLH转录因子

Full-Length Transcriptome Sequencing of Under Simulated Feeding Highlights bHLH Transcription Factor Involved in Defense Response.

作者信息

Wen Quanmin, Cui Yajie, Xu Tian, Deng Yadi, Hao Dejun, Chen Ruixu

机构信息

Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry and Grassland, Nanjing Forestry University, Nanjing 210037, China.

School of Landscape Architecture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212499, China.

出版信息

Plants (Basel). 2025 Jul 3;14(13):2038. doi: 10.3390/plants14132038.

DOI:10.3390/plants14132038

PMID:40648048

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12251683/

Abstract

BACKGROUND

is a significant lipid-producing tree species in China and a susceptible host for both the pine wood nematode and its insect vector, . The basic helix-loop-helix (bHLH) family of transcription factors play a crucial role in responding to both biotic and abiotic stresses. However, the role of bHLH in terpene-induced defense in remains poorly studied.

RESULTS

Transcriptome sequencing using DNA Nanoball Sequencing (DNBSEQ) and PacBio Sequel platforms was performed, revealing differences in gene expression in . branch under the simulated feeding treatment of methyl jasmonate (MeJA) spraying. Fifteen genes were cloned and analyzed, among which eight highly upregulated genes showed similar temporal expression after MeJA treatment and . adult feeding. Five highly upregulated genes with nuclear localization were highly expressed in after feeding and interacted with the promoter of the terpene synthase gene , confirming their involvement in the defense response of against the adult feeding.

CONCLUSIONS

Our results unveil the temporal changes and the regulation of the induced defense system in mediated by both MeJA signaling and feeding treatment. The potential application for transgenic experiments and the breeding of resistant species in the future were discussed.

摘要

背景

是中国一种重要的产脂树种，也是松材线虫及其昆虫传播媒介的易感寄主。碱性螺旋-环-螺旋（bHLH）转录因子家族在应对生物和非生物胁迫中起关键作用。然而，bHLH在萜烯诱导的防御中的作用仍研究不足。

结果

使用DNA纳米球测序（DNBSEQ）和PacBio Sequel平台进行转录组测序，揭示了在茉莉酸甲酯（MeJA）喷雾模拟取食处理下枝条中的基因表达差异。克隆并分析了15个基因，其中8个高度上调的基因在MeJA处理和成虫取食后表现出相似的时间表达。5个具有核定位的高度上调基因在成虫取食后在中高度表达，并与萜烯合酶基因的启动子相互作用，证实它们参与了对成虫取食的防御反应。