• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

金丝桃苷对 Ang II 介导的 bEnd.3 细胞凋亡及体外血脑屏障模型损伤的保护作用。

The protective effects of hyperoside on Ang II-mediated apoptosis of bEnd.3 cells and injury of blood-brain barrier model in vitro.

机构信息

Department of Neurology, Jiangsu Province Hospital of Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China.

Department of Neurology, Xuzhou City Hospital of Traditional Chinese Medicine, Xuzhou, Jiangsu Province, China.

出版信息

BMC Complement Med Ther. 2022 Jun 13;22(1):157. doi: 10.1186/s12906-022-03635-9.

DOI:10.1186/s12906-022-03635-9
PMID:35698113
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9195266/
Abstract

BACKGROUND

Hypertension and its associated dysfunction of the blood-brain barrier (BBB) are considered to contribute to cerebral small vessel disease (cSVD). Angiotensin II (Ang II), as an important vasoactive peptide of the renin-angiotensin system (RAS), is not only a pivotal molecular signal in hypertension, but also causes BBB leakage, cSVD and its related cognitive impair. Hyperoside (Hyp), a flavone glycoside, has antioxidant, antiphlogistic and anti-apoptosis effects. In this study, we investigate the protection of Hyp on apoptosis of bEnd.3 cells and BBB disruption in vitro induced by Ang II.

METHODS

We used bEnd.3 cells to imitate a BBB monolayer model and explored the protection of Hyp on Ang II-induced BBB leakage. The apoptotic activity was assessed by TUNEL staining and flow cytometry. The expression of apoptosis pathway related proteins, tight junction proteins and transcytosis related proteins were detected by western blot assay. The BBB model permeability was detected through measuring the flux of sodium fluorescein (Na-F).

RESULTS

We found that Hyp can not only effectively inhibit the apoptosis of bEnd.3 induced by Ang II, but also protect the structural soundness and functional integrity of BBB model by affecting the expression levels of junctional adhesion molecule A (JAM-A), Claudin-5, zonula occludens-1 (ZO-1), Caveolin-1 (Cav-1) and major facilitator superfamily domain-containing protein 2a (Mfsd2a).

CONCLUSION

Hyp might be a potent compound for preventing Ang II-induced BBB disruption.

摘要

背景

高血压及其相关的血脑屏障（BBB）功能障碍被认为是导致脑小血管病（cSVD）的原因。血管紧张素 II（Ang II）作为肾素-血管紧张素系统（RAS）的一种重要血管活性肽，不仅是高血压的关键分子信号，还会导致 BBB 渗漏、cSVD 及其相关认知障碍。金丝桃苷（Hyp）是一种黄酮糖苷，具有抗氧化、抗炎和抗凋亡作用。在这项研究中，我们研究了 Hyp 对 Ang II 诱导的 bEnd.3 细胞体外凋亡和 BBB 破坏的保护作用。

方法

我们使用 bEnd.3 细胞模拟 BBB 单层模型，并探讨了 Hyp 对 Ang II 诱导的 BBB 渗漏的保护作用。通过 TUNEL 染色和流式细胞术评估细胞凋亡活性。通过 Western blot 检测凋亡途径相关蛋白、紧密连接蛋白和转胞吞作用相关蛋白的表达。通过测量荧光素钠（Na-F）的通量来检测 BBB 模型通透性。

结果

我们发现 Hyp 不仅能有效抑制 Ang II 诱导的 bEnd.3 细胞凋亡，还能通过影响紧密连接蛋白 A（JAM-A）、Claudin-5、闭合蛋白-1（ZO-1）、窖蛋白-1（Cav-1）和主要易化超家族域蛋白 2a（Mfsd2a）的表达水平来保护 BBB 模型的结构完整性和功能完整性。

结论

Hyp 可能是一种预防 Ang II 诱导的 BBB 破坏的有效化合物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be39/9195266/450f19265c3b/12906_2022_3635_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be39/9195266/bd59ac462c29/12906_2022_3635_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be39/9195266/ac753943c351/12906_2022_3635_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be39/9195266/a2a5af77c11a/12906_2022_3635_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be39/9195266/255afeb59c28/12906_2022_3635_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be39/9195266/2c1b3d303887/12906_2022_3635_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be39/9195266/24526ebb1397/12906_2022_3635_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be39/9195266/450f19265c3b/12906_2022_3635_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be39/9195266/bd59ac462c29/12906_2022_3635_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be39/9195266/ac753943c351/12906_2022_3635_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be39/9195266/a2a5af77c11a/12906_2022_3635_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be39/9195266/255afeb59c28/12906_2022_3635_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be39/9195266/2c1b3d303887/12906_2022_3635_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be39/9195266/24526ebb1397/12906_2022_3635_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be39/9195266/450f19265c3b/12906_2022_3635_Fig7_HTML.jpg

相似文献

1
The protective effects of hyperoside on Ang II-mediated apoptosis of bEnd.3 cells and injury of blood-brain barrier model in vitro.金丝桃苷对 Ang II 介导的 bEnd.3 细胞凋亡及体外血脑屏障模型损伤的保护作用。
BMC Complement Med Ther. 2022 Jun 13;22(1):157. doi: 10.1186/s12906-022-03635-9.
2
The protective effect of harpagoside on angiotensin II (Ang II)-induced blood-brain barrier leakage in vitro.哈巴俄苷对血管紧张素 II（Ang II）诱导的体外血脑屏障渗漏的保护作用。
Phytother Res. 2021 Nov;35(11):6241-6254. doi: 10.1002/ptr.7269. Epub 2021 Sep 5.
3
Catalpol relieved angiotensin II-induced blood-brain barrier destruction via inhibiting the TLR4 pathway in brain endothelial cells.梓醇通过抑制脑内皮细胞 TLR4 通路缓解血管紧张素 II 诱导的血脑屏障破坏。
Pharm Biol. 2022 Dec;60(1):2210-2218. doi: 10.1080/13880209.2022.2142801.
4
Effects of methyl-beta-cyclodextrin on blood-brain barrier permeability in angiotensin II-induced hypertensive rats.甲基-β-环糊精对血管紧张素Ⅱ诱导的高血压大鼠血脑屏障通透性的影响。
Brain Res. 2019 Jul 15;1715:148-155. doi: 10.1016/j.brainres.2019.03.024. Epub 2019 Mar 23.
5
Mfsd2a (Major Facilitator Superfamily Domain Containing 2a) Attenuates Intracerebral Hemorrhage-Induced Blood-Brain Barrier Disruption by Inhibiting Vesicular Transcytosis.Mfsd2a（含主要易化子超家族结构域2a）通过抑制囊泡转胞吞作用减轻脑出血诱导的血脑屏障破坏。
J Am Heart Assoc. 2017 Jul 19;6(7):e005811. doi: 10.1161/JAHA.117.005811.
6
Pharmacological depletion of microglia and perivascular macrophages prevents Vascular Cognitive Impairment in Ang II-induced hypertension.药理耗尽小胶质细胞和血管周巨噬细胞可预防血管性认知障碍在血管紧张素 II 诱导的高血压。
Theranostics. 2020 Jul 25;10(21):9512-9527. doi: 10.7150/thno.44394. eCollection 2020.
7
Ang-(1-7) exerts protective role in blood-brain barrier damage by the balance of TIMP-1/MMP-9.血管紧张素-(1-7)通过 TIMP-1/MMP-9 的平衡发挥对血脑屏障损伤的保护作用。
Eur J Pharmacol. 2015 Feb 5;748:30-6. doi: 10.1016/j.ejphar.2014.12.007. Epub 2014 Dec 15.
8
Selective sphingosine-1-phosphate receptor 1 modulator attenuates blood-brain barrier disruption following traumatic brain injury by inhibiting vesicular transcytosis.选择性鞘氨醇-1-磷酸受体 1 调节剂通过抑制囊泡转胞吞作用来减轻创伤性脑损伤后的血脑屏障破坏。
Fluids Barriers CNS. 2022 Jul 11;19(1):57. doi: 10.1186/s12987-022-00356-6.
9
Effects of atorvastatin on blood-brain barrier permeability during L-NAME hypertension followed by angiotensin-II in rats.阿托伐他汀对L-硝基精氨酸甲酯诱导高血压后再给予血管紧张素-II的大鼠血脑屏障通透性的影响。
Brain Res. 2005 May 3;1042(2):184-93. doi: 10.1016/j.brainres.2005.02.044.
10
Specific role of tight junction proteins claudin-5, occludin, and ZO-1 of the blood-brain barrier in a focal cerebral ischemic insult.血脑屏障紧密连接蛋白 Claudin-5、Occludin 和 ZO-1 在局灶性脑缺血损伤中的特定作用。
J Mol Neurosci. 2011 Jun;44(2):130-9. doi: 10.1007/s12031-011-9496-4. Epub 2011 Feb 12.

引用本文的文献

1
Cardiovascular protective effects of natural flavonoids on intestinal barrier injury.天然黄酮类化合物对肠道屏障损伤的心血管保护作用。
Mol Cell Biochem. 2025 Jan 17. doi: 10.1007/s11010-025-05213-2.
2
Interaction of major facilitator superfamily domain containing 2A with the blood-brain barrier.含2A的主要易化子超家族结构域与血脑屏障的相互作用。
Neural Regen Res. 2025 Aug 1;20(8):2133-2152. doi: 10.4103/NRR.NRR-D-24-00191. Epub 2024 Sep 6.
3
GSK3β Substrate-competitive Inhibitors Regulate the gut Homeostasis and Barrier Function to Inhibit Neuroinflammation in Scopolamine-induced Alzheimer's Disease Model Mice.

本文引用的文献

1
Flavonoids-rich extract from Bidens bipinnata L. protects pancreatic β-cells against oxidative stress-induced apoptosis through intrinsic and extrinsic pathways.富含三叶鬼针草（Bidens bipinnata L.）的类黄酮提取物通过内源性和外源性途径保护胰岛β细胞免受氧化应激诱导的细胞凋亡。
J Ethnopharmacol. 2021 Jul 15;275:114097. doi: 10.1016/j.jep.2021.114097. Epub 2021 Apr 3.
2
Serum metabonomics coupled with HPLC-LTQ/orbitrap MS and multivariate data analysis on the ameliorative effects of Bidens bipinnata L. in hyperlipidemic rats.血清代谢组学结合 HPLC-LTQ/orbitrap MS 和多元数据分析研究三叶鬼针草对高血脂大鼠的改善作用。
J Ethnopharmacol. 2020 Nov 15;262:113196. doi: 10.1016/j.jep.2020.113196. Epub 2020 Jul 27.
3
GSK3β底物竞争性抑制剂调节肠道稳态和屏障功能以抑制东莨菪碱诱导的阿尔茨海默病模型小鼠的神经炎症。
Inflammation. 2024 Aug 24. doi: 10.1007/s10753-024-02133-z.
4
Hyperoside Nanomicelles Alleviate Atherosclerosis by Modulating the Lipid Profile and Intestinal Flora Structure in High-Fat-Diet-Fed Apolipoprotein-E-Deficient Mice.金丝桃苷纳米胶束通过调节高脂饮食载脂蛋白 E 缺陷小鼠的血脂谱和肠道菌群结构来缓解动脉粥样硬化。
Molecules. 2023 Jun 29;28(13):5088. doi: 10.3390/molecules28135088.
Effects of angiotensin-II on brain endothelial cell permeability via PPARalpha regulation of para- and trans-cellular pathways.
血管紧张素-II 通过 PPARα 对细胞旁和细胞转运途径的调节作用对脑内皮细胞通透性的影响。
Brain Res. 2019 Nov 1;1722:146353. doi: 10.1016/j.brainres.2019.146353. Epub 2019 Jul 26.
4
Risk Factors for and Clinical Relevance of Incident and Progression of Cerebral Small Vessel Disease Markers in an Asian Memory Clinic Population.亚洲记忆门诊人群中脑小血管病标志物的发生和进展的危险因素及其临床意义。
J Alzheimers Dis. 2019;67(4):1209-1219. doi: 10.3233/JAD-180911.
5
Blood Pressure Gradients in the Brain: Their Importance to Understanding Pathogenesis of Cerebral Small Vessel Disease.大脑中的血压梯度：它们对理解脑小血管病发病机制的重要性。
Brain Sci. 2019 Jan 23;9(2):21. doi: 10.3390/brainsci9020021.
6
High Systolic Blood Pressure Induces Cerebral Microvascular Endothelial Dysfunction, Neurovascular Unit Damage, and Cognitive Decline in Mice.高收缩压可导致小鼠脑血管内皮功能障碍、神经血管单元损伤和认知功能下降。
Hypertension. 2019 Jan;73(1):217-228. doi: 10.1161/HYPERTENSIONAHA.118.12048.
7
Cerebral small vessel disease: neuroimaging markers and clinical implication.脑小血管病：神经影像学标志物及其临床意义。
J Neurol. 2019 Oct;266(10):2347-2362. doi: 10.1007/s00415-018-9077-3. Epub 2018 Oct 5.
8
Hyperoside protects the blood-brain barrier from neurotoxicity of amyloid beta 1-42.金丝桃苷可保护血脑屏障免受β-淀粉样蛋白1-42的神经毒性作用。
Neural Regen Res. 2018 Nov;13(11):1974-1980. doi: 10.4103/1673-5374.239445.
9
Hypertension-induced cognitive impairment: insights from prolonged angiotensin II infusion in mice.高血压导致的认知障碍：来自于小鼠中延长血管紧张素 II 输注的研究结果。
Hypertens Res. 2018 Oct;41(10):817-827. doi: 10.1038/s41440-018-0090-9. Epub 2018 Aug 17.
10
Blood-Brain Barrier Permeability Is Regulated by Lipid Transport-Dependent Suppression of Caveolae-Mediated Transcytosis.血脑屏障通透性受脂转运依赖性小窝介导的转胞吞作用抑制调控。
Neuron. 2017 May 3;94(3):581-594.e5. doi: 10.1016/j.neuron.2017.03.043. Epub 2017 Apr 13.