Liu Xin, Chen Longyu, Zhang Minghu, Li Hui, Jiang Xiaomei, Zhang Junqing, Jia Zhenjiao, Ma Pan, Hao Ming, Jiang Bo, Huang Lin, Ning Shunzong, Yuan Zhongwei, Chen Xuejiao, Chen Xue, Liu Dengcai, Zhang Lianquan
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.
Plant Dis. 2023 Jan;107(1):125-130. doi: 10.1094/PDIS-04-22-0865-RE. Epub 2023 Jan 19.
(2n = 2x = 14, AA) is an important relative of wheat. This species tolerates many different types of environmental stresses, including drought, salt, and pathogenic infection, and is lower in dietary fiber and higher in antioxidants, protein (15 to 18%), lipids, and trace elements than common wheat. However, the gene transfer rate from this species to common wheat is low, and few species-specific molecular markers are available. In this study, the wheat- substitution line Z1889, derived from a cross between the common wheat cultivar Crocus and line G52, was identified using multicolor fluorescence in situ hybridization, multicolor genomic in situ hybridization, and a 55K single-nucleotide polymorphism array. Z1889 was revealed to be a 4A (4B) substitution line with a high degree of resistance to stripe rust pathogen strains prevalent in China. In addition, 22 4A chromosome-specific molecular markers and 11 genome-specific molecular markers were developed from 1,145 4A chromosome-specific fragments by comparing the sequences generated by specific-length amplified fragment sequencing, with an efficiency of up to 55.0%. Furthermore, the specificity of these markers was verified in four species containing the A genome. These markers not only can be used for the detection of the 4A chromosome but also provide a basis for molecular marker-assisted, selection-based breeding in wheat.
（2n = 2x = 14，AA）是小麦的一个重要近缘种。该物种能耐受多种不同类型的环境胁迫，包括干旱、盐胁迫和病原体感染，并且与普通小麦相比，其膳食纤维含量较低，抗氧化剂、蛋白质（15%至18%）、脂质和微量元素含量较高。然而，该物种向普通小麦的基因转移率较低，且可用的物种特异性分子标记很少。在本研究中，利用多色荧光原位杂交、多色基因组原位杂交和55K单核苷酸多态性阵列，鉴定了由普通小麦品种藏红花与G52品系杂交得到的小麦代换系Z1889。结果表明，Z1889是一个4A（4B）代换系，对中国流行的条锈病菌株具有高度抗性。此外，通过比较特定长度扩增片段测序产生的序列，从1145个4A染色体特异性片段中开发出22个4A染色体特异性分子标记和11个基因组特异性分子标记，效率高达55.0%。此外，在四个含有A基因组的物种中验证了这些标记的特异性。这些标记不仅可用于检测4A染色体，还为小麦分子标记辅助选择育种提供了依据。
