Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, China.
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.
J Appl Genet. 2020 May;61(2):169-177. doi: 10.1007/s13353-020-00553-9. Epub 2020 Feb 18.
Diploid wild einkorn wheat, Triticum boeoticum Boiss (AA, 2n = 2x = 14), is a wheat-related species with a blue aleurone layer. In this study, six blue-grained wheat lines were developed from F progeny of crosses between common wheat and T. boeoticum. The chromosome constitutions of these lines were characterized by fluorescence in situ hybridization (FISH) using the oligonucleotide probes Oligo-pTa535-1, Oligo-pSc119.2-1, Oligo-pTa71-2, and (AAC). Multicolor FISH using Oligo-pTa535-1, Oligo-pSc119.2-1, and Oligo-pTa71-2 identified all 42 common wheat chromosomes, while Oligo-pTa535-1 and (AAC) discriminated the 14 chromosomes of T. boeoticum. FISH revealed that all six blue-grained lines were wheat-T. boeoticum 4A (4B) disomic substitution lines. The substitution lines were validated by genotyping using the wheat 55 K single nucleotide polymorphism (SNP) array containing 53,063 markers. These 4A (4B) substitution lines represent novel germplasm for blue-grained wheat breeding. The FISH probes and SNP markers used here can be applied in the development of blue-grained wheat-Triticum boeoticum translocation lines.
二倍体野生一粒小麦，Triticum boeoticum Boiss（AA，2n=2x=14），是一个与小麦相关的种，具有蓝色糊粉层。本研究以普通小麦与 T. boeoticum 的杂交 F1 后代为材料，选育出 6 个蓝粒小麦品系。这些品系的染色体组成通过荧光原位杂交（FISH）技术，使用寡核苷酸探针 Oligo-pTa535-1、Oligo-pSc119.2-1、Oligo-pTa71-2 和（AAC）进行鉴定。多色 FISH 结合 Oligo-pTa535-1、Oligo-pSc119.2-1 和 Oligo-pTa71-2 可以鉴定出所有 42 条普通小麦染色体，而 Oligo-pTa535-1 和（AAC）可以区分 14 条 T. boeoticum 染色体。FISH 结果显示，这 6 个蓝粒品系均为小麦-一粒小麦 4A（4B）二体代换系。通过使用包含 53063 个标记的小麦 55K 单核苷酸多态性（SNP）芯片进行基因分型，验证了这些代换系的真实性。这些 4A（4B）代换系代表了蓝粒小麦的新型种质资源。本研究中使用的 FISH 探针和 SNP 标记可应用于蓝粒小麦-小麦易位系的开发。
