Plant Breeding Institute, The University of Sydney, Cobbitty, NSW, 2570, Australia.
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, China.
Theor Appl Genet. 2020 Apr;133(4):1095-1107. doi: 10.1007/s00122-020-03534-y. Epub 2020 Jan 18.
A physical map of Secale cereale chromosome 6R was constructed using deletion mapping, and a new stripe rust resistance gene Yr83 was mapped to the deletion bin of FL 0.73-1.00 of 6RL. Rye (Secale cereale L., RR) possesses valuable genes for wheat improvement. In the current study, we report a resistance gene conferring stripe rust resistance effective from seedling to adult plant stages located on chromosome 6R. This chromosome was derived from triticale line T-701 and also carries highly effective resistance to the cereal cyst nematode species Heterodera avenae Woll. A wheat-rye 6R(6D) disomic substitution line exhibited high levels of seedling resistance to Australian pathotypes of the stripe rust (Puccinia striiformis f. sp. tritici; Pst) pathogen and showed an even greater resistance to the Chinese Pst pathotypes in the field. Ten chromosome 6R deletion lines and five wheat-rye 6R translocation lines were developed earlier in the attempt to transfer the nematode resistance gene to wheat and used herein to map the stripe rust resistance gene. These lines were subsequently characterized by sequential multicolor fluorescence in situ hybridization (mc-FISH), genomic in situ hybridization (GISH), mc-GISH, PCR-based landmark unique gene (PLUG), and chromosome 6R-specific length amplified fragment sequencing (SLAF-Seq) marker analyses to physically map the stripe rust resistance gene. The new stripe rust resistance locus was located in a chromosomal bin with fraction length (FL) 0.73-1.00 on 6RL and was named Yr83. A wheat-rye translocation line T6RL (#5) carrying the stripe rust resistance gene will be useful as a new germplasm in breeding for resistance.
利用缺失作图构建了黑麦 6R 染色体的物理图谱，并将一个新的条锈病抗性基因 Yr83 定位到 6RL 的 FL 0.73-1.00 缺失-bin 中。黑麦（Secale cereale L.，RR）拥有可用于改良小麦的宝贵基因。在本研究中，我们报告了一个位于 6R 染色体上的有效控制幼苗至成株期条锈病的抗性基因。该染色体源自黑小麦品种 T-701，还携带对麦长管蚜物种 Heterodera avenae Woll 的高度有效抗性。一个小麦-黑麦 6R(6D) 二体替换系在幼苗期对澳大利亚条锈病（Puccinia striiformis f. sp. tritici；Pst）病原菌表现出高水平的抗性，并且在田间对中国 Pst 病原菌表现出更强的抗性。为了将线虫抗性基因转移到小麦中，我们早期开发了 10 个 6R 缺失系和 5 个小麦-黑麦 6R 易位系，并在此用于定位条锈病抗性基因。随后，通过顺序多色荧光原位杂交（mc-FISH）、基因组原位杂交（GISH）、mc-GISH、基于 PCR 的标记独特基因（PLUG）和 6R 特异性长度扩增片段测序（SLAF-Seq）标记分析对这些系进行了特征描述，以对条锈病抗性基因进行物理作图。新的条锈病抗性基因位于 6RL 上的分长度（FL）0.73-1.00 染色体-bin 中，被命名为 Yr83。携带条锈病抗性基因的小麦-黑麦易位系 T6RL（#5）将作为一种新的抗性遗传资源，在小麦育种中具有应用价值。
