National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Shaanxi Normal University, Xi'an 710119, China; Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry (Shaanxi Normal University), The Ministry of Education, Xi'an 710119, China; College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China.
National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Shaanxi Normal University, Xi'an 710119, China; Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry (Shaanxi Normal University), The Ministry of Education, Xi'an 710119, China; College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China.
Carbohydr Polym. 2022 Sep 1;291:119524. doi: 10.1016/j.carbpol.2022.119524. Epub 2022 Apr 25.
Polysaccharide is one of the main active components of Polygonatum sibiricum. For this study, P. sibiricum polysaccharides (PSP) were obtained through purification using DEAE-Cellulose52 and Sephacryl G-150 column chromatography. The obtained samples were named PSP1, PSP2 and PSP3. The PSP1 sample was found to have the highest content and the best solubility, and a subsequent. So, its structure and characterization were analyzed. The main sugar residue linkages were found to be → 1)-β-D-Fruf-(2 → 1)-β-D-Fruf-(2 → 1), 1 → -β-D-Fruf-(2 → 6)α-D-Glcp (1→, →4)-β-D-Manp-(1 → 4)-β-D-Manp-(1→ and →6)-β-D-Glcp-(1 → 4)-β-D-Manp-(1→ link existed. Branch chain analysis indicated →1,6)-β-D-Fruf-(2→, β-D-Fruf-(2→, →1,6)-β-D-Fruf-(2→, →6)-β-D- Fruf-(2→ link existed, and the link site was at position C-6. In vitro antioxidant activity tests showed that PSP1 had a certain scavenging effect on DPPH, hydroxyl radical, superoxide anion radical and a particular effect on the chelating ability of ferrous. This suggested that P. sibiricum polysaccharides may be a potential antioxidant.
多糖是黄精的主要活性成分之一。在这项研究中，通过 DEAE-纤维素 52 和 Sephacryl G-150 柱层析对黄精多糖（PSP）进行了纯化。得到的样品分别命名为 PSP1、PSP2 和 PSP3。结果发现 PSP1 样品含量最高，溶解度最好，因此对其结构和性质进行了分析。结果表明，其主要糖残基连接键为→1）-β-D-Fruf-（2→1）-β-D-Fruf-（2→1），1→-β-D-Fruf-（2→6）α-D-Glcp（1→，→4）-β-D-Manp-（1→4）-β-D-Manp-（1→和→6）-β-D-Glcp-（1→4）-β-D-Manp-（1→连接。支链分析表明，→1，6）-β-D-Fruf-（2→，β-D-Fruf-（2→，→1，6）-β-D-Fruf-（2→，→6）-β-D-Fruf-（2→连接存在，连接位点在 C-6 位。体外抗氧化活性测试表明，PSP1 对 DPPH、羟基自由基、超氧阴离子自由基具有一定的清除作用，对亚铁离子的螯合能力有特殊作用。这表明黄精多糖可能是一种潜在的抗氧化剂。
