伊拉克巴比伦山羊中山羊关节炎脑炎病毒分子流行率的首次研究。
First study on the molecular prevalence of caprine arthritis encephalitis virus in goats in Babylon, Iraq.
作者信息
Mosa Ahmed Hamzah, Hamzah Karrar Jasim, Aljabory Hamed A H
机构信息
Department of Internal and Preventive Veterinary Medicine, College of Veterinary Medicine, AL-Qasim Green University, Babylon, Iraq.
出版信息
Vet World. 2022 Apr;15(4):1129-1133. doi: 10.14202/vetworld.2022.1129-1133. Epub 2022 Apr 28.
BACKGROUND AND AIM
Caprine arthritis encephalitis virus (CAEV) is a virus that affects goats all over the world and causes enormous economic losses; as a result, screening for the disease is a priority, especially in Iraq. The present study aimed to estimate the prevalence of CAEV in infected goats using the précised PCR method in Babylon, Iraq.
MATERIALS AND METHODS
A total of 85 blood samples from goats aged 1 month to ≥6 years were analyzed for CAEV infections using molecular methods. The polymerase chain reaction primer was designed to amplify a 573 bp region of the proviral pol gene.
RESULTS
The CAEV tests revealed that five out of 85 goats were positive for CAEV. There were no significant differences in CAEV infection according to goat sex and significant differences according to age.
CONCLUSION
Based on these results, the present study is the first molecular survey to confirm the current CAEV genome in an Iraqi goat flock.
背景与目的
山羊关节炎脑炎病毒（CAEV）是一种影响全球山羊并造成巨大经济损失的病毒；因此，对该疾病进行筛查是当务之急，尤其是在伊拉克。本研究旨在利用精确PCR方法估计伊拉克巴比伦地区感染山羊中CAEV的流行率。
材料与方法
使用分子方法对85份来自1月龄至≥6岁山羊的血液样本进行CAEV感染分析。设计聚合酶链反应引物以扩增前病毒pol基因的573 bp区域。
结果
CAEV检测显示，85只山羊中有5只CAEV呈阳性。CAEV感染在山羊性别方面无显著差异，在年龄方面有显著差异。
结论
基于这些结果，本研究是首次在伊拉克山羊群中确认当前CAEV基因组的分子调查。
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