Shahandeh Erfan, Ghorbani Mahboubeh, Mokhlesabadifarahani Tahereh, Bardestani Fateme
Department of Biology, Islamic Azad University of Hamedan, Hamedan, Iran.
Department of Midwifery North Khorasan University of Medical Sciences, Bojnurd, Iran.
Clin Exp Reprod Med. 2022 Jun;49(2):87-92. doi: 10.5653/cerm.2022.05267. Epub 2022 May 31.
The aim of this study was to determine the effects of melatonin and selenium in freezing extenders on frozen-thawed rat sperm.
Semen samples were collected from 20 adult male Wistar albino rats. Following dilution, the samples were divided into six groups: four cryopreserved groups with 1 mM and 0.5 mM melatonin and selenium supplements, and two fresh and cryopreserved control groups. The rapid freezing technique was used to freeze the samples. Flow cytometry was used to assess plasma membrane integrity, mitochondrial membrane potential, and DNA damage, while computer-assisted sperm analysis was used to assess motility.
Total motility was higher in the 1 mM melatonin supplementation group than in the cryopreserved control group (mean±standard error of the mean, 69.89±3.05 vs. 59.21±1.31; p≤0.05). The group with 1 mM selenium had the highest plasma membrane integrity (42.35%±1.01%). The cryopreserved group with 0.5 mM selenium had the highest mitochondrial membrane potential, whereas the cryopreserved control group had the lowest (45.92%±4.53% and 39.45%±3.52%, respectively).
Cryopreservation of rat semen supplemented with 1 mM melatonin increased sperm motility after freeze-thawing, while supplementation with 0.5 mM selenium increased mitochondrial activity.
本研究旨在确定冷冻稀释液中褪黑素和硒对冻融大鼠精子的影响。
从20只成年雄性Wistar白化大鼠采集精液样本。稀释后，将样本分为六组：四组冷冻保存组，分别添加1 mM和0.5 mM的褪黑素和硒，以及两组新鲜和冷冻保存对照组。采用快速冷冻技术冷冻样本。使用流式细胞术评估质膜完整性、线粒体膜电位和DNA损伤，同时使用计算机辅助精子分析评估活力。
1 mM褪黑素补充组的总活力高于冷冻保存对照组（平均值±平均标准误差，69.89±3.05对59.21±1.31；p≤0.05）。1 mM硒组的质膜完整性最高（42.35%±1.01%）。0.5 mM硒冷冻保存组的线粒体膜电位最高，而冷冻保存对照组最低（分别为45.92%±4.53%和39.45%±3.52%）。
添加1 mM褪黑素冷冻保存大鼠精液可提高冻融后精子活力，而添加0.5 mM硒可提高线粒体活性。
