Institute of Chemistry, UNICAMP, PO Box 6154, 13083-970, Campinas, Brazil.
Institute of Analytical and Bioanalytical Chemistry, Ulm University, 89081, Ulm, BW, Germany.
Anal Methods. 2022 Jul 14;14(27):2657-2664. doi: 10.1039/d2ay00377e.
A THz-TDS spectrometer (terahertz time-domain spectroscopy) with ASOPS technology (asynchronous optical sampling) was employed in this study, aiming to explore the potential of the technique and to develop analytical applications for the identification of some saccharides. Analytical curves with linear responses were obtained in the saccharide concentration range from 1.7 to 11.7 (/). The characteristic peaks used for each sugar for univariate calibrations were fructose (1.704 THz), sucrose (1.827 THz), glucose (1.435 THz), lactose (1.373 THz), saccharin (2.664 THz), and sucralose (2.219 THz). Limits of detection around 1.0% (/) were obtained. Gaussian peak fitting was also employed as a tool to aid in the identification of saccharide components in mixtures and it was observed that the region between 0.5 and 2.0 THz is more adequate for such analysis since scattering is less evident. It was observed that in a mixture of sucrose, glucose and lactose (5% or 10% (/) each) in the range of 1.31 to 1.51 THz some sub-peaks of pure lactose and glucose can be identified.
本研究采用太赫兹时域光谱仪（太赫兹时域光谱技术）与异步光学采样技术（ASOPS），旨在探索该技术的潜力，并开发用于鉴定一些糖类的分析应用。在 1.7 到 11.7 （/）的糖浓度范围内，得到了具有线性响应的分析曲线。对于单变量校准，每个糖的特征峰分别为果糖（1.704 THz）、蔗糖（1.827 THz）、葡萄糖（1.435 THz）、乳糖（1.373 THz）、糖精（2.664 THz）和三氯蔗糖（2.219 THz）。检测限约为 1.0%（/）。还采用高斯峰拟合作为一种工具，以帮助鉴定混合物中的糖成分，观察到 0.5 到 2.0 THz 之间的区域更适合这种分析，因为散射不太明显。在 1.31 到 1.51 THz 的范围内，观察到蔗糖、葡萄糖和乳糖（每种 5%或 10%（/））的混合物中，可以识别出一些纯乳糖和葡萄糖的次峰。
