Department of Animal Reproduction, National Institute for Agricultural and Food Research and Technology (INIA-CSIC), Madrid, Spain.
Department of Animal Pathology, Veterinary Faculty of Zaragoza, Zaragoza, Spain.
Reprod Domest Anim. 2022 Oct;57 Suppl 5(Suppl 5):90-93. doi: 10.1111/rda.14179. Epub 2022 Jun 20.
The aim was to assess the reproductive efficiency of different techniques used to preserve spermatozoa in artificial insemination semen doses (AI-doses) by evaluating refrigeration at 15°C, cryopreservation and encapsulation. Forty-two hyperprolific sows were treated with buserelin and inseminated once at a single fixed time. The fertility rate, embryonic vesicles viability and the early embryonic mortality (arrested conceptuses) evaluated post-mortem at 24th day of pregnancy, were analysed in order to assess the effectiveness of each proposed technique. Results show an overall reduction on fertility using the three proposal sperm preservation techniques (69.27%, 60.00% and 78.75% for refrigerated, frozen-thawed and encapsulated AI-doses, respectively). Total number of embryonic vesicles was very similar among the three treatments; yet, the number of viable vesicles was numerically different among groups, and thus, embryonic viability was 79.25%, 80.0% and 87.15% for refrigerated, frozen-thawed and encapsulated AI-doses, respectively.
目的是评估在人工授精精液剂量（AI 剂量）中使用不同技术保存精子的生殖效率，评估 15°C 冷藏、冷冻保存和包埋。42 头高产母猪用布舍瑞林处理，并在单一固定时间进行一次授精。通过评估死后 24 天的受孕率、胚胎泡活力和早期胚胎死亡率（停滞胚胎），分析了每种拟议技术的有效性。结果表明，使用三种精子保存技术（冷藏、冷冻解冻和包埋 AI 剂量的受孕率分别为 69.27%、60.00%和 78.75%），整体受孕率降低。三种处理方式的胚胎泡总数非常相似；然而，活胚胎泡的数量在各组之间存在差异，因此，冷藏、冷冻解冻和包埋 AI 剂量的胚胎存活率分别为 79.25%、80.0%和 87.15%。
