College of Chemistry and Materials Science, Huaibei Normal University, 235000 Huaibei, PR China.
College of Life Sciences, Huaibei Normal University, 235000, Huaibei, PR China.
Int J Syst Evol Microbiol. 2022 Jun;72(6). doi: 10.1099/ijsem.0.005436.
A Gram-stain-negative, non-spore-forming and rod-shaped bacterium, designated strain NS-102, was isolated from herbicide-contaminated soil sampled in Nanjing, PR China, and its taxonomic status was investigated by a polyphasic approach. Cell growth of strain NS-102 occurred at 16-42 °C (optimum, 30 °C), at pH 5.0-8.0 (optimum, pH 6.0) and in the presence of 0-3.5 % (w/v) NaCl (optimum, without addition of NaCl). The 16S rRNA gene sequence of strain NS-102 shows high similarity to that of YJ03 (96.9 % similarity), followed by T16R-129 (93.8 %) and QH (93.6 %). Average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the draft genomes of strain NS-102 and YJ03 were 72.5, 69.4 and 18.6%, respectively. The only respiratory quinone was MK-7, and phosphatidylethanolamine and unidentified lipids were the major polar lipids. The major cellular fatty acids of strain NS-102 contained high amounts of iso-C (24.6 %), iso-C3-OH (24.1 %), iso-C G (16.6 %) and summed feature 3 (C 6 and/or C 7) (15.6 %). The G+C content of the total DNA was determined to be 40.0 mol%. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain NS-102 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NS-102 (=CCTCC AB 2017249=KCTC 62322).
一株革兰氏阴性、不产芽孢、杆状的细菌，菌株 NS-102，从中国南京受除草剂污染的土壤中分离得到，通过多相分类学方法对其分类地位进行了研究。菌株 NS-102 的细胞生长发生在 16-42°C（最佳温度为 30°C），在 pH 值 5.0-8.0（最佳 pH 值为 6.0），在 0-3.5%（w/v）NaCl（最佳情况下不加 NaCl）存在的条件下。菌株 NS-102 的 16S rRNA 基因序列与 YJ03（相似度为 96.9%）、T16R-129（相似度为 93.8%）和 QH（相似度为 93.6%）最为相似。菌株 NS-102 的基因组草图与 YJ03 的平均核苷酸同一性、平均氨基酸同一性和数字 DNA-DNA 杂交值分别为 72.5%、69.4%和 18.6%。唯一的呼吸醌是 MK-7，磷脂酰乙醇胺和未鉴定的脂质是主要的极性脂质。菌株 NS-102 的主要细胞脂肪酸含有大量的异-C（24.6%）、异-C3-OH（24.1%）、异-C G（16.6%）和总和特征 3（C 6 和/或 C 7）（15.6%）。总 DNA 的 G+C 含量为 40.0mol%。形态学、生理学、化学分类学和系统发育分析清楚地区分了该菌株与其最接近的系统发育近邻。因此，菌株 NS-102 代表了一个新的属种，提议命名为 sp. nov.。模式菌株为 NS-102（=CCTCC AB 2017249=KCTC 62322）。
