Chen Qing, Zang Xiao-Xia, Hang Xing, Chen Jing, Wang Hong-Mei, Li Shun-Peng, Jiang Jian-Dong, Chen Kai
Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
College of Life Sciences, Zaozhuang University, Zaozhuang, 277160, People's Republic of China.
Antonie Van Leeuwenhoek. 2017 Aug;110(8):1061-1068. doi: 10.1007/s10482-017-0879-6. Epub 2017 Apr 28.
Strain C3-5, a Gram-negative, asporogenous, rod-shaped bacterium, was isolated from a tetrabromobisphenol A contaminated soil. Growth was observed at 10-37 °C (optimum 30 °C) and at pH 5.5-9.5 (optimum pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain C3-5 is a member of the genus Terrimonas and exhibits high sequence similarities with Terrimonas pekingensis QH (99.0%) and Terrimonas rhizosphaerae CR94 (97.3%), and exhibits low (<97%) sequence similarities with other known Terrimonas species. Chemotaxonomic analysis revealed that strain C3-5 possesses menaquinone-7 (MK-7) as the major isoprenoid quinone and iso-C, iso-C G, iso-C 3-OH and summed feature 3 (C ω6c and/or C ω7c) as the major (>5% of total) fatty acids. The polar lipids were determined to be a lipid, glycolipid, phospholipid, phosphoaminolipid and phosphatidylethanolamine. The DNA G+C content was found to be 42.6 mol%. The DNA-DNA relatedness values with the closely related strains T. pekingensis QH and T. rhizosphaerae CR94 were 25.2 and 48.5%, respectively. Based on phylogenetic analysis, phenotypic characteristics and chemotaxonomic data, strain C3-5 is considered to represent a novel species of the genus Terrimonas, for which the name Terrimonas suqianensis sp. nov. is proposed. The type strain is C3-5 (= CCTCC AB 2017042 = KCTC 52676).
