Department of Hematology, Faculty of Medicine, School of Health Sciences, University of Ioannina, Greece.
Department of Pathology, University Hospital of Saint-Etienne, France.
Pathol Res Pract. 2022 Aug;236:153964. doi: 10.1016/j.prp.2022.153964. Epub 2022 Jun 1.
Autophagy and cellular senescence are interrelated cellular stress responses important for cellular homeostasis and they have been implicated in the pathogenesis of classical Hodgkin lymphoma (cHL). However, the presence of autophagy and cellular senescence and their relation with clinical and laboratory parameters needs further elucidation. Thus, autophagy (LC3B and p62 immunohistochemical expression) and cellular senescence (p16 immunohistochemical expression and SenTraGor™ staining) were studied in tissue sections from 59 patients with cHL. Autophagy and cellular senescence-associated markers were detected in variable proportions of Hodgkin and Reed-Sternberg (HRS) cells in cHL cases. High nuclear p62 immunohistochemical expression in HRS cells showed significant positive correlation with relapse of the disease (p = 0.037). Heterogeneous autophagy and cellular senescence patterns were revealed in HRS cells suggesting biological heterogeneity of cHL. The detection of high nuclear p62 expression in HRS cells may identify a subset of cHL with more aggressive clinical behavior.
自噬和细胞衰老都是细胞应激反应的重要过程，对细胞内环境的稳定起着重要作用，并且与经典霍奇金淋巴瘤（cHL）的发病机制有关。然而，自噬和细胞衰老的存在及其与临床和实验室参数的关系仍需要进一步阐明。因此，本研究检测了 59 例 cHL 患者组织切片中的自噬（LC3B 和 p62 的免疫组化表达）和细胞衰老（p16 的免疫组化表达和 SenTraGor™染色）。在 cHL 病例中，发现霍奇金和里德-斯特恩伯格（HRS）细胞中存在不同比例的自噬和细胞衰老相关标志物。HRS 细胞核 p62 免疫组化高表达与疾病复发呈显著正相关（p=0.037）。HRS 细胞中自噬和细胞衰老的异质性提示 cHL 存在生物学异质性。HRS 细胞核中 p62 高表达的检测可能识别出具有更具侵袭性临床行为的 cHL 亚群。
