Chen Xiaoju, Jiang Xiaohong, Lu Yanyan, Yao Yien, Lu Jiali, Zhi Qiang, Lai Lejin, Liang Jie, Li Chaoqian
Department of General Internal Medicine, Affiliated Tumor Hospital of Guangxi Medical University Nanning 530021, Guangxi Province, China.
Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University Nanning 530021, Guangxi Province, China.
Am J Transl Res. 2022 May 15;14(5):3430-3438. eCollection 2022.
To investigate whether inhalation affects asthma via γδ T cell regulation.
Forty male Balb/c mice were randomly divided into 4 groups: normal group, asthma group, control group and intervention group. The normal group was given no treatment. For the asthma group, control group and intervention group, the mice were sensitized and stimulated with ovalbumin (OVA) to establish asthma models. Mice in the asthma group were not treated. Mice in the control group were treated with γδ T cell suspension from normal mice, and those in the intervention group were treated with γδ T cell suspension from mice intervened by Mycobacterium bovis. ELISA assay was adopted for quantification of IL-4 and IFN-γ in mouse alveolar fluid (BALF), and flow cytometry for determining the percentage of IL-4 and IFN-γ from mononuclear cells of lung tissues.
The airway responsiveness of the asthma group was higher than that of the normal group. The degree of airway inflammation in the intervention group was lighter than that in the control group, and it was significantly alleviated compared with the asthma group (P<0.05). Compared with the asthma group, the level of IL-4 in the BALF of the control group and the intervention group decreased significantly, while the level of IFN-γ increased significantly (P<0.05). Compared with the control group, the level of IL-4 in the BALF of the intervention group was significantly lower, while the level of IFN-γ was significantly higher (P<0.05). In addition, the results of flow cytometry were basically consistent with the results of Elisa.
Aerosol inhalation of Mycobacterium bovis can alleviate the Th2 dominant immune response induced by OVA sensitization and regulate the Th1/Th2 immune imbalance in patients with asthma.
探讨吸入是否通过γδT细胞调节影响哮喘。
将40只雄性Balb/c小鼠随机分为4组：正常组、哮喘组、对照组和干预组。正常组不做处理。对于哮喘组、对照组和干预组，用卵清蛋白（OVA）对小鼠进行致敏和激发以建立哮喘模型。哮喘组小鼠不做处理。对照组小鼠用正常小鼠的γδT细胞悬液处理，干预组小鼠用经牛分枝杆菌干预的小鼠的γδT细胞悬液处理。采用ELISA法对小鼠肺泡灌洗液（BALF）中的IL-4和IFN-γ进行定量，采用流式细胞术测定肺组织单个核细胞中IL-4和IFN-γ的百分比。
哮喘组的气道反应性高于正常组。干预组的气道炎症程度轻于对照组，与哮喘组相比明显减轻（P<0.05）。与哮喘组相比，对照组和干预组BALF中IL-4水平显著降低，而IFN-γ水平显著升高（P<0.05）。与对照组相比，干预组BALF中IL-4水平显著降低，而IFN-γ水平显著升高（P<0.05）。此外，流式细胞术结果与ELISA结果基本一致。
雾化吸入牛分枝杆菌可减轻OVA致敏诱导的Th2主导的免疫反应，调节哮喘患者Th1/Th2免疫失衡。
