Department of Dermatology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China.
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China.
Mol Med Rep. 2022 Aug;26(2). doi: 10.3892/mmr.2022.12771. Epub 2022 Jun 15.
Hypoxia‑related long non‑coding RNAs (lncRNAs) are important indicators of the poor prognosis of cancers. The present study aimed to explore the potential relationship between melanoma and hypoxia‑related lncRNAs. The transcriptome and clinical data of patients with melanoma were downloaded from The Cancer Genome Atlas database. The prognostic hypoxia‑related lncRNAs were screened out using Pearson's correlation test and univariate Cox analysis. As a result, a hypoxia‑related‑lncRNA signature based on the expression of 7 lncRNAs was constructed, with one unfavourable [MIR205 host gene (MIR205HG)] and six favourable (T cell receptor β variable 11‑2, HLA‑DQB1 antisense RNA 1, AL365361.1, AC004847.1, ubiquitin specific peptidase 30 antisense RNA 1 and AC022706.1) lncRNAs as prognostic factors for melanoma. Patients with melanoma were divided into high‑ and low‑risk groups based on the risk score obtained. Survival analyses were performed to assess the prognostic value of the present risk model. Potential tumour‑associated biological pathways associated with the present signature were explored using gene set enrichment analysis. The CIBERSORT algorithm demonstrated the important role of the hypoxia‑related lncRNAs in regulating tumour‑infiltrating immune cells. Clinical samples collected from our center partly confirmed our findings. Cell Counting Kit‑8 and flow cytometry assays indicated the suppression of proliferation of melanoma cells following inhibition of MIR205HG expression. Indicators of the canonical Wnt/β‑catenin signalling pathway were detected by western blotting. The present study demonstrated that MIR205HG could promote melanoma cell proliferation partly via the canonical Wnt/β‑catenin signalling pathway. These findings indicated a 7‑hypoxia‑related‑lncRNA signature that can serve as a novel predictor of melanoma prognosis.
缺氧相关长非编码 RNA（lncRNA）是癌症预后不良的重要指标。本研究旨在探讨黑色素瘤与缺氧相关 lncRNA 之间的潜在关系。从癌症基因组图谱数据库中下载黑色素瘤患者的转录组和临床数据。使用 Pearson 相关检验和单因素 Cox 分析筛选出与预后相关的缺氧 lncRNA。结果构建了一个基于 7 个 lncRNA 表达的缺氧相关 lncRNA 特征，其中一个不利的[miR205 宿主基因（MIR205HG）]和六个有利的（T 细胞受体β可变 11-2、HLA-DQB1 反义 RNA 1、AL365361.1、AC004847.1、泛素特异性肽酶 30 反义 RNA 1 和 AC022706.1）lncRNA 作为黑色素瘤的预后因素。根据获得的风险评分将黑色素瘤患者分为高风险和低风险组。进行生存分析以评估本风险模型的预后价值。使用基因集富集分析探索与本特征相关的潜在肿瘤相关生物学途径。CIBERSORT 算法表明，缺氧相关 lncRNA 在调节肿瘤浸润免疫细胞方面发挥着重要作用。从我们中心收集的临床样本部分证实了我们的发现。细胞计数试剂盒-8 和流式细胞术检测表明，抑制 MIR205HG 表达可抑制黑色素瘤细胞的增殖。通过蛋白质印迹法检测经典 Wnt/β-连环蛋白信号通路的标志物。本研究表明，MIR205HG 可通过经典 Wnt/β-连环蛋白信号通路部分促进黑色素瘤细胞的增殖。这些发现表明，一个 7-缺氧相关-lncRNA 特征可作为黑色素瘤预后的新预测指标。
