Universidade Federal do Ceará - UFC, Faculty of Pharmacy, Dentistry and Nursing , Department of Clinical Dentistry , Fortaleza , CE , Brazil .
Universidade Federal do Ceará - UFC. School of Dentistry at Sobral , Department of Dentistry , Sobral , CE , Brazil .
Braz Oral Res. 2022 Jun 10;36:e077. doi: 10.1590/1807-3107bor-2022.vol36.0077. eCollection 2022.
Gene polymorphisms can predispose to periodontal disease, as demonstrated by the well-documented association between aggressive periodontitis and single nucleotide polymorphisms (SNPs) such as rs153745 in the GLT6D1 gene and rs3217992 in the CDKN2BAS gene. The purpose of this study was to evaluate the presence of these SNPs in Brazilian patients with advanced periodontitis (stages III/IV, Grade B/C) vs. healthy controls. A total of 100 patients with periodontitis (Group BC) were enrolled. Of these, 51 patients were classified as stage III and 49 patients were classified as stage IV, and 52 were Grade B (Group B) and 48 were Grade C (Group C). The control Group consisted of 61 healthy subjects. DNA samples extracted from buccal epithelial cells were used to genotype the SNPs rs1537415 (GLT6D1) and rs3217992 (CDKN2BAS) by real-time quantitative PCR. No significant differences in polymorphism frequency were found between the control Group and each of the patient groups (BC, B, or C), and Group B did not differ from Group C. In conclusion, the evaluated SNPs had no significant influence on the prevalence of periodontal disease in the sampled Brazilian population.
基因多态性可能导致牙周病，侵袭性牙周炎与 GLT6D1 基因中的 rs153745 和 CDKN2BAS 基因中的 rs3217992 等单核苷酸多态性（SNP）的明确关联证明了这一点。本研究旨在评估这些 SNP 在巴西晚期牙周炎（III/IV 期，B/C 级）患者与健康对照组中的存在情况。共纳入 100 例牙周炎患者（BC 组）。其中，51 例患者为 III 期，49 例为 IV 期，52 例为 B 级（B 组），48 例为 C 级（C 组）。对照组由 61 名健康受试者组成。从颊上皮细胞中提取 DNA 样本，通过实时定量 PCR 对 SNP rs1537415（GLT6D1）和 rs3217992（CDKN2BAS）进行基因分型。在对照组和每个患者组（BC、B 或 C）之间，以及 B 组和 C 组之间，多态性频率均无显著差异。综上所述，在所研究的巴西人群中，评估的 SNP 对牙周病的患病率没有显著影响。
