Department of Periodontics, Government Dental College, Medical College, Post Office, Calicut, Kerala 673008, India.
J Periodontol. 2010 Jan;81(1):62-9. doi: 10.1902/jop.2009.090256.
Interleukin (IL)-1beta gene polymorphisms are considered a potential risk factor for periodontal disease. The aim of this study is to identify the association of IL-1beta gene polymorphisms with chronic periodontitis and aggressive periodontitis in a Malayalam-speaking Dravidian population from South India.
The case-control study consisted of 43 patients with chronic periodontitis and 54 patients with aggressive periodontitis as cases, and the control group consisted of 101 healthy subjects. All subjects were genotyped for IL-1beta +3954, -511, and -31 loci by polymerase chain reaction amplification followed by restriction enzyme digestion and gel electrophoresis. Genotype, allele, and haplotype analyses were done.
Analyses for allele and genotypes showed a high frequency of the C allele and CC genotype for single nucleotide polymorphism IL-1beta +3954 in the group with chronic periodontitis and no difference for patients with aggressive periodontitis compared to controls (P <0.05). Haplotype analysis showed that IL-1beta -31 and -511 were in strong linkage disequilibrium in all groups. The IL-1beta -31 allele T was in linkage with allele T of IL-1beta +3954 in the control group.
In the Malayalam-speaking Dravidian population, allele C of IL-1beta +3954 appeared to be an important risk factor for chronic periodontitis. The IL-1beta -31 allele T was in linkage with allele T of IL-1beta +3954 in the control group. No gene polymorphisms were found in patients with aggressive periodontitis. More studies with a larger sample size involving the entire cluster of the IL-1beta gene are necessary to determine the exact role of IL-1beta gene polymorphisms in periodontal disease.
白细胞介素（IL）-1β基因多态性被认为是牙周病的潜在危险因素。本研究旨在确定白细胞介素-1β基因多态性与南印度德拉威语人群慢性牙周炎和侵袭性牙周炎的关系。
这项病例对照研究包括 43 例慢性牙周炎患者和 54 例侵袭性牙周炎患者作为病例，对照组由 101 名健康受试者组成。所有受试者均采用聚合酶链反应扩增后限制性内切酶消化和凝胶电泳法检测白细胞介素-1β+3954、-511 和-31 基因座的基因型。进行了基因型、等位基因和单倍型分析。
等位基因和基因型分析显示，慢性牙周炎组中单核苷酸多态性白细胞介素-1β+3954 的 C 等位基因和 CC 基因型频率较高，而侵袭性牙周炎患者与对照组相比无差异（P<0.05）。单倍型分析显示，白细胞介素-1β-31 和-511 在所有组中均存在强连锁不平衡。白细胞介素-1β-31 等位基因 T 与对照组白细胞介素-1β+3954 的等位基因 T 连锁。
在德拉威语人群中，白细胞介素-1β+3954 的 C 等位基因似乎是慢性牙周炎的一个重要危险因素。白细胞介素-1β-31 等位基因 T 与对照组白细胞介素-1β+3954 的等位基因 T 连锁。侵袭性牙周炎患者未发现基因多态性。需要进行更大样本量的研究，涉及整个白细胞介素-1β基因簇，以确定白细胞介素-1β基因多态性在牙周病中的确切作用。
