Lallinger G J, Reiner S L, Cooke D W, Toffaletti D L, Perfect J R, Granger D L, Durack D T
Infect Immun. 1987 May;55(5):1289-93. doi: 10.1128/iai.55.5.1289-1293.1987.
Naegleria fowleri meningoencephalitis is usually fatal in humans despite treatment. As a new approach, we tested intracisternal passive immune therapy in rabbits with amebic meningoencephalitis by using antinaegleria immune serum, an immunoglobulin G fraction, and a newly developed monoclonal antibody to N. fowleri. Both the immune serum and an immunoglobulin G fraction isolated from it by affinity chromatography provided a consistent, although temporary, protective effect, shown by prolongation of survival (P = 0.001). Multiple doses of immune serum further prolonged survival (P = 0.005). The protective effect of serum was retained after heating to 56 degrees C. We then developed a monoclonal antibody to N. fowleri which provided similar protection. Passive intracisternal antibody therapy might serve as an adjunctive component in the treatment of amebic meningoencephalitis.
福氏耐格里阿米巴脑膜炎即便经过治疗，在人类中通常也是致命的。作为一种新方法，我们通过使用抗耐格里阿米巴免疫血清、一种免疫球蛋白G组分以及一种新研发的针对福氏耐格里阿米巴的单克隆抗体，对患有阿米巴脑膜炎的兔子进行了脑池内被动免疫治疗。免疫血清以及通过亲和层析从其中分离出的免疫球蛋白G组分均提供了一种持续的（尽管是暂时的）保护作用，表现为存活时间延长（P = 0.001）。多剂量的免疫血清进一步延长了存活时间（P = 0.005）。血清在加热至56摄氏度后仍保留其保护作用。然后我们研发了一种针对福氏耐格里阿米巴的单克隆抗体，其提供了类似的保护作用。脑池内被动抗体治疗可能作为阿米巴脑膜炎治疗中的一种辅助成分。
