Zhang L, Marciano-Cabral F, Bradley S G
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond 23298-0110.
Antimicrob Agents Chemother. 1988 Jul;32(7):962-5. doi: 10.1128/AAC.32.7.962.
Mice challenged intranasally with Naegleria fowleri died of primary amoebic meningoencephalitis. Mice given 30 mg of cyclophosphamide per kg of body weight daily for 10 days starting 2 days before challenge were protected. Neither cyclophosphamide nor serum from cyclophosphamide-treated mice inhibited N. fowleri in vitro. A metabolic product of cyclophosphamide, acrolein, inhibited growth and enflagellation of N. fowleri. Acrolein at 40 microM was amoebicidal. Acrolein injured starved cells and amoebae at 5 degrees C and growing N. fowleri.
经鼻内接种福氏耐格里阿米巴的小鼠死于原发性阿米巴脑膜脑炎。在接种前2天开始，每天给小鼠按每千克体重30毫克的剂量注射环磷酰胺，持续10天，这些小鼠受到了保护。环磷酰胺以及经环磷酰胺处理的小鼠血清在体外均不能抑制福氏耐格里阿米巴。环磷酰胺的一种代谢产物丙烯醛可抑制福氏耐格里阿米巴的生长和鞭毛形成。40微摩尔的丙烯醛具有杀阿米巴作用。丙烯醛可损伤处于5摄氏度饥饿状态的细胞和阿米巴以及生长中的福氏耐格里阿米巴。
