Akasaki M, Ishizaka K
Int Arch Allergy Appl Immunol. 1987;82(3-4):417-8. doi: 10.1159/000234243.
Immunization with alum-absorbed ovalbumin (OA) failed to induce IgE antibody responses in SJL mice, while mice pretreated with either cyclophosphamide (CY) or gamma-irradiation prior to immunization transiently formed IgE antibodies. Spleen cells of OA-primed SJL mice formed IgE-suppressive factors upon incubation with OA. In contrast, spleen cells of the CY-treated or irradiated mice formed IgE-potentiating factors. It was found that CY treatment diminished the formation of glycosylation inhibiting factor (GIF) and enhanced the formation of glycosylation enhancing factor (GEF). The results suggest that the enhancement of the IgE antibody response by CY treatment is due not only to elimination of suppressor T cells but also to the activation of GEF-forming lymphocytes.
用明矾吸附的卵清蛋白（OA）免疫未能在SJL小鼠中诱导IgE抗体反应，而在免疫前用环磷酰胺（CY）或γ射线预处理的小鼠则短暂形成了IgE抗体。用OA免疫的SJL小鼠的脾细胞在与OA孵育后形成了IgE抑制因子。相反，经CY处理或照射的小鼠的脾细胞形成了IgE增强因子。研究发现，CY处理减少了糖基化抑制因子（GIF）的形成并增强了糖基化增强因子（GEF）的形成。结果表明，CY处理增强IgE抗体反应不仅是由于抑制性T细胞的消除，还由于形成GEF的淋巴细胞的激活。
