Carswell F, Mukherjee S, Heap P
Int Arch Allergy Appl Immunol. 1987;82(3-4):465-7. doi: 10.1159/000234255.
Inbred rats were sensitized by intraperitoneal injection of DNP19 ovalbumin (DNP-OA) or saline. The rats were subsequently challenged by exposure to an aerosol of DNP-OA or bovine gamma globulins (BGG) for 10 min. Tracheal tissue was processed immediately after challenge in fixative containing 1% lanthanum. Planimetry of transmission electron micrographs showed 59% of the intraepithelial membranes were stained with lanthanum in the DNP-OA-sensitized and DNP-OA-challenged group; 39% in the saline-sensitized, DNP-OA-challenged group, and 26% in the DNP-OA-sensitized, BGG-challenged group. X-ray static probe microanalysis confirmed that most lanthanum penetrated in the DNP-OA-sensitized and DNP-OA-challenged group. In this group, the magnitude of the mechanical response, measured by body plethysmography, correlated directly (r = 0.74) with the lanthanum penetration. Our results confirm that increased permeability of the intercellular spaces occurs in immediate pulmonary hypersensitivity. They suggest that this change in permeability may be involved in producing the mechanical response.
近交系大鼠通过腹腔注射二硝基苯基-19-卵白蛋白（DNP-OVA）或生理盐水致敏。随后，让大鼠暴露于DNP-OVA或牛γ球蛋白（BGG）气雾剂中10分钟进行激发。激发后立即将气管组织置于含1%镧的固定剂中处理。透射电子显微镜照片的平面测量显示，在DNP-OVA致敏并接受DNP-OVA激发的组中，59%的上皮内细胞膜被镧染色；在生理盐水致敏、DNP-OVA激发的组中为39%，在DNP-OVA致敏、BGG激发的组中为26%。X射线静态探针微分析证实，在DNP-OVA致敏并接受DNP-OVA激发的组中，大多数镧穿透了细胞膜。在该组中，通过体容积描记法测量的机械反应强度与镧的穿透呈直接正相关（r = 0.74）。我们的结果证实，在速发型肺超敏反应中，细胞间间隙的通透性增加。结果表明，这种通透性变化可能与产生机械反应有关。
