Kepron W, Jackson C J, Sehon A H
Int Arch Allergy Appl Immunol. 1987;82(3-4):468-70. doi: 10.1159/000234256.
Newborn mongrel dogs were sensitized with conjugates of ovalbumin (OA) and 2,4-dinitrophenol (OA-DNP3) in the presence of Al(OH)3 to produce high levels of anti-OA and anti-DNP IgE antibody. At 4-6 months of age, when anti-DNP and anti-OA antibody levels reached titers of 64 by passive cutaneous anaphylaxis, the dogs underwent separate inhalation and intravenous challenges with conjugates of DNP and bovine gamma globulin (DNP15-BGG) and OA. Inhalation challenge with DNP15-BGG and OA resulted in 5- and 10-fold increases in airflow resistance, respectively. Intravenous challenge with either DNP15-BGG or OA produced profound anaphylaxis with 60-80% decreases in blood pressure, cardiac output and regional blood flows in the carotid, superior mesenteric and renal arteries, and the distal aorta. Treatment of sensitized dogs with 5 doses of 20 mg of conjugates of DNP and polyvinyl alcohol (DNP2-PVA) on alternate days resulted in suppression of anti-DNP IgE antibody production; abrogation of established airway and vascular anaphylactic sensitivities; no change in regional blood flows, and no effect on sensitivities to challenge with OA.
新生杂种犬在氢氧化铝存在的情况下，用卵清蛋白（OA）与2,4 -二硝基苯酚的偶联物（OA - DNP3）进行致敏，以产生高水平的抗OA和抗DNP IgE抗体。在4至6个月龄时，当通过被动皮肤过敏反应抗DNP和抗OA抗体水平达到效价64时，对犬分别进行DNP与牛γ球蛋白的偶联物（DNP15 - BGG）和OA的吸入和静脉激发试验。用DNP15 - BGG和OA进行吸入激发试验分别导致气流阻力增加5倍和10倍。用DNP15 - BGG或OA进行静脉激发试验会引发严重过敏反应，导致颈动脉、肠系膜上动脉、肾动脉和主动脉远端的血压、心输出量和局部血流量降低60% - 80%。对致敏犬隔日给予5剂20 mg的DNP与聚乙烯醇的偶联物（DNP2 - PVA）进行治疗，结果抑制了抗DNP IgE抗体的产生；消除了已建立的气道和血管过敏敏感性；局部血流量无变化，并且对OA激发试验的敏感性没有影响。
